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Human primary myotubes +/- electrical pulse stimulation
Electrical pulse stimulation (EPS) is used to study exercise-related adaptations in muscle cells in vitro. We compared the effects of two EPS protocols: 1. intermittent high-frequency or 2. continuous low-frequency stimulation, on glucose and fatty acid metabolism, muscle fiber-type markers, mitocho...
ORGANISM(S): Homo sapiens (Human) 
2026-01-12 | PXD059967 | Pride
We hypothesized that muscle contraction produces a cellular stress signal capable to increase lipolysis to sustain fuel availability during exercise. The aim of the present study was to identify novel exercise-regulated myokines, aka exerkines, able to promote lipolysis in human adipocytes. To this ...
ORGANISM(S): Homo sapiens (Human) 
2020-05-26 | PXD014126 | Pride
Lifestyle disorders like obesity, type 2 diabetes (T2D), and cardiovascular diseases can be prevented and treated by regular physical activity. During exercise, skeletal muscles release signaling factors that communicate with other organs and mediate beneficial effects of exercise. These factors inc...
ORGANISM(S): Homo sapiens (Human) 
2023-03-17 | PXD040935 | Pride
RNA-Seq of Human LHCN-M2 skeletal muscle myotubes to Electrical Pulse Stimulation
Study on changes in gene expression in primary cultures of neonatal rat ventricular cardiomyocytes to electric stimulation. Through comparing non-stimulated, stimulated and blebbistatin supplemented and stimulated cultures we set out to identify the genes that are specifically activated by electric ...
ORGANISM(S): Rattus norvegicus 
Analysis of the effect of electrical field stimulation frequency at the gene expression level. Electrical stimulation has been shown to mature nascent cardiomyocytes and alter their beating properties. The purpose of the array was to identify potential mediators of these effects. Total RNA was isola...
ORGANISM(S): Homo sapiens 
Expression profiling of a cellular exercise model based on the electrical pulse stimulation.
Human myogenic progenitor cells (MPCs) were isolated from the gracilis of two patients (1 female and 1 male) undergoing anterior cruciate ligament reconstruction (age 30-34 years) using a human anti-CD56 (1:20, Cat# 355503, Biolegend) antibody for FACS. Cells were passaged 2-3 times in growth media ...
ORGANISM(S): Homo sapiens 
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