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ProteomicsMapping physiological ADP-ribosylation using Activated Ion Electron Transfer Dissociation (AI-ETD)
ADP-ribosylation (ADPr) is a post-translational modification that plays pivotal roles in a wide range of cellular processes. Mass spectrometry (MS)-based analysis of ADPr under physiological conditions, without relying on genetic or chemical perturbation, has been hindered by technical limitations. ...
ORGANISM(S): Homo sapiens (Human) 
2020-09-23 | PXD017417 | Pride
Activated ion electron transfer dissociation Post-translational modification Adpr Ptm Parp Ai-etd Adp-ribosylation 
ProteomicsTop-down detection of oxidative protein footprinting by Collision-Induced Dissociation, Electron-Transfer Dissociation and Electron-Capture Dissociation
Fast photochemical oxidation of proteins (FPOP) footprinting is a structural mass spectrometry method that maps proteins by fast and irreversible chemical reactions. The position of oxidative modification reflects solvent accessibility and site reactivity and thus provides information about protein ...
ORGANISM(S): Bos taurus (Bovine) Equus caballus (Horse) 
2023-07-20 | PXD031926 | Pride
Electron-transfer dissociation Fast photochemical oxidation of proteins Top-down Collision-induced dissociation Mass spectrometry. Electron-capture dissociation 
ProteomicsMS characterization of spidroin-1
For MS characterization of major ampullate spidroin-1 from the three species of Nephila clavipes, edulis and madagascariensis, an experimental approach was developed combining 2-DE with multiple proteolytic in-gel digestion (trypsin, chymotrypsin, pepsin, proteinase K, subtilisin and proteinase 1...
ORGANISM(S): Nephila clavipes (Golden silk orbweaver) 
2022-02-22 | PXD000057 | Pride
Major ampullate silk Nephila sp. Post-translational modifications Phosphorylation Nanolc-esi-cid/etd-ms/ms Spidroin-1 
ProteomicsCapillary zone electrophoresis-tandem mass spectrometry with activated ion electron transfer dissociation for large-scale top-down proteomics
Capillary zone electrophoresis (CZE)-tandem mass spectrometry (MS/MS) has been recognized as an efficient approach for top-down proteomics recently for its high-capacity separation and highly sensitive detection of proteoforms. However, the commonly used collision-based dissociation methods often ca...
ORGANISM(S): Escherichia coli 
2019-06-11 | PXD012247 | Pride
Top-down proteomics E. coli Cze-ms Ai-etd 
ProteomicsElectron-based dissociation is needed for O-glycopeptides derived from OpeRATOR proteolysis
The recently described O-glycoprotease OpeRATOR presents exciting opportunities for O-glycoproteomics. This bacterial enzyme purified from A. muciniphila cleaves N-terminally to serine and threonine residues that are modified with (preferably asialylated) O-glycans, providing orthogonal cleavage rel...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2020-11-03 | PXD020077 | Pride
Glycoproteomics O-glycosites Operator Protease Electron transfer dissociation 
ProteomicsUrinary polypeptide ETD/CID analysis, part 2
Urine as a biofluid is commonly used in clinical diagnostics, including those performed during pregnancy. Urine is a rich source of polypeptides and protein degradation products, which have been filtered from blood plasma, thus urine has strong potential as a source for novel clinical diagnostics in...
ORGANISM(S): Homo sapiens (Human) 
2016-02-15 | PXD002346 | Pride
Urine Pregnancy Non-tryptic peptides Peptidome Electron transfer dissociation 
ProteomicsUrinary polypeptide ETD/CID analysis, part 3
Urine as a biofluid is commonly used in clinical diagnostics, including those performed during pregnancy. Urine is a rich source of polypeptides and polypeptidic protein degradation products, which have been filtered from blood plasma, thus urine has potential as a source for novel clinical diagnost...
ORGANISM(S): Homo sapiens (Human) 
2016-02-15 | PXD002372 | Pride
Urine Pregnancy Non-tryptic peptides Peptidome Electron transfer dissociation 
ProteomicsGlycoproteomics with AI-ETD - Capturing site-specific heterogeneity with large-scale N-glycoproteome analysis
Protein glycosylation is a highly important, yet a poorly understood protein post-translational modification. Thousands of possible glycan structures and compositions create potential for tremendous site heterogeneity and analytical challenge. A lack of suitable analytical methods for large-scale an...
ORGANISM(S): Mus musculus (Mouse) 
2019-04-02 | PXD011533 | Pride
Glycosylation Electron transfer dissociation Glycopeptides 
ProteomicsMirroring the charged termini; ETD/HCD fragmentation characteristics of LysargiNase and tryptic peptides and their benefits for peptide sequencing in proteomics
A key step in proteomics is the digestion of proteins into peptides, so far largely done by using trypsin. Tryptic digestion leads to peptides that in ESI-MS attain predominantly two charges, via protonation at the free N-terminus and at the C-terminal basic residue Arginine or Lysine. These peptide...
ORGANISM(S): Homo sapiens (Human) 
2016-12-02 | PXD004447 | Pride
Fragmentation Etd Hcd Lysarginase Orbitrap Phosphoproteomics Trypsin 
ProteomicsChemical labeling for fine mapping of IgG N-glycosylation by ETD-MS
Immunoglobulin G (IgG), which contains four subclasses (IgG1-4), is one of the most important class of glycoproteins in immune system. Because of its importance in immune system, a steady increase of interest in developing IgG as biomarker or biotherapeutic agents for the treatment of diseases has b...
ORGANISM(S): Homo sapiens (Human) 
2019-08-26 | PXD014713 | Pride
Chemical labeling Igg Glycosylation Glycopeptides Electron transfer dissociation (etd) mass spectrometry 
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