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ProteomicsComparing the diagnostic classification accuracy of iTRAQ, peak-area, spectral-counting and emPAI methods for relative quantification in expression proteomics
We present a comparison of the diagnostic accuracy of iTRAQ with three label free methods (peak-area, spectral-counting, and emPAI) for relative quantification using a spiked proteome standard.
ORGANISM(S): Equus Caballus (ncbitaxon:9796) Bos Taurus (ncbitaxon:9913) Escherichia Coli (ncbitaxon:562) Oryctolagus Cuniculus (ncbitaxon:9986) Gallus Gallus (ncbitaxon:9031) Saccharomyces Cerevisiae (ncbitaxon:4932) 
2016-06-08 | MSV000079803 | MassIVE
UnknownemPAI-assisted strategy enhances screening and assessment of Mycobacterium tuberculosis infection serological markers.
Not available
S-EPMC8313264 | biostudies-literature
UnknownSemiquantitative analysis of clinical heat stress in Clostridium difficile strain 630 using a GeLC/MS workflow with emPAI quantitation.
Not available
S-EPMC3933415 | biostudies-literature
ProteomicsEvaluating Protein Enrichment Methods to Improve Biomarker Discovery in Equine Neurological Disorders
This study evaluated and compared three sample preparation strategies for proteomic analysis of equine cerebrospinal fluid (CSF): native in-solution digestion, the ProteoMiner™ Small-Capacity Enrichment Kit (Bio-Rad®), and the PreOmics Enrich-iST™ Kit (PreOmics®). CSF is in direct contact with the c...
ORGANISM(S): Equus caballus (Horse) 
2026-04-13 | PXD063239 | Pride
Cerebrospinal fluid (csf)  proteomics  native digestion  proteominer™ small-capacity enrichment kit  preomics enrich-ist™  liquid chromatography with tandem mass spectrometry (lc-ms/ms) 
UnknownUrinary Proteomic Shifts over Time and Their Associations with eGFR Decline in Chronic Kidney Disease.
Not available
S-EPMC11762955 | biostudies-literature
ProteomicsIdentification of new proteins related with cisplatin resistance in S. cerevisiae
The aim of this study is to select a cisplatin resistant Saccharomyces cerevisiae strain as a model organism to look for new molecular markers of cisplatin resistance and the identification of mechanisms/interactions involved. A cisplatin resistant S. cerevisiae strain was obtained after continuous ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-02-25 | PXD020665 | Pride
Yeast Resistance Saccharomyces cerevisiae Cisplatin Proteomic 
UnknownUrinary Protein Profiling for Potential Biomarkers of Chronic Kidney Disease: A Pilot Study.
Not available
S-EPMC9689510 | biostudies-literature
ProteomicsCharacterisation of nuclear and sub-nuclear proteomes of Saccharomyces cerevisiae
This study concerns characterisation of the nuclear, chromatin and nuclear non-chromatin proteomes of the yeast Saccharomyces cerevisiae. We performed nuclear and chromatin enrichments from exponentially growing cells using differential centrifugation and analysed the nuclear and chromatin pellets, ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2019-11-07 | PXD007525 | Pride
Shotgun proteomics Organelle Nucleus Saccharomyces cerevisiae Chromatin Lc-ms/ms 
ProteomicsDistinct proteomic profile of extracellular vesicles from peritoneal exudates from WT and CD38-deficient mice 2 weeks after pristane injection (lupus disease model)
Extracellular vesicles (EVs) are present in all body fluids. Shed by cells, their molecular make-up reflects that of their cell of origin and/or tissue pathological situation. Our working hypothesis was that analyzing the protein composition, protein abundance, and functional clustering of EVs relea...
ORGANISM(S): Mus musculus (Mouse) 
2022-10-20 | PXD035257 | Pride
Mouse  extracellular vesicles  peritoneal exudate cells  lc-msms  quantitative proteomics 
ProteomicsImmunomic identification of antigens
Immunomic screening of mice immune sera with different protection efficiencies against the whole P. yoelii 17X parasite proteome was performed to identify the repertoire of antigens recognized by those sera. The calculation of weighted scores reflecting the likelihood of protection of each antigens ...
ORGANISM(S): Plasmodium yoelii 17X 
2019-02-15 | PXD010567 | Pride
Malaria Immunoprecipitation 2d western blot Empai Lc-ms/ms 
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