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MetabolomicsComparison of popular enrichment methods for untargeted in vitro metabolomics.

Introduction

Untargeted metabolomics is a popular method by which researchers measure a large portion of the metabolites present in a biological system at once. This approach usually results in complex data sets containing tens to hundreds of thousands of observations whi...

2025-08-11 | MTBLS12332 | MetaboLights
MetabolomicsIlluminating the dark space of neutral glycosphingolipidome by selective enrichment and profiling at multi-structural levels

Glycosphingolipids (GSLs) are essential components of cell membranes, particularly enriched in the nervous system. Altered molecular distributions of GSLs are increasingly associated with human diseases, emphasizing the significance of lipidomic profiling. Traditional GSL analysis methods are ham...

2024-06-25 | MTBLS10400 | MetaboLights
MetabolomicsStreamlined identification of strain engineering targets for bioprocess improvement using metabolic pathway enrichment analysis
Metabolomics is a powerful tool for the identification of genetic targets for bioprocess optimisation. However, in most cases, only the biosynthetic pathway directed to product formation is analysed, limiting the identification of these targets. Some studies have used untargeted metabolomics, allowi...
2023-08-31 | MTBLS6667 | MetaboLights
MetabolomicsEvaluation of extraction solvents for untargeted metabolomics analysis of enrichment reactor cultures performing enhanced biological phosphorus removal (EBPR)

INTRODUCTION: The extraction solvent mixtures were optimized for untargeted metabolomics analysis of microbial communities from two laboratory scale activated sludge reactors performing enhanced biological phosphorus removal (EBPR).

OBJECTIVE: To develop a ...

2019-08-16 | MTBLS884 | MetaboLights
MetabolomicsHelicobacter pylori infection promotes TMAO accumulation through Escherichia coli enrichment with gut–liver circadian disruption and hepatic metabolic injury
Helicobacter pylori (H. pylori) infection is associated with metabolic dysfunction-associated steatotic liver disease, but the microbial changes and circadian disturbances accompanying distal hepatic injury remain unclear. In specific pathogen-free (SPF) mice exposed to dietary choline, H. pylori in...
2026-09-18 | MTBLS15673 | MetaboLights
MetabolomicsPositional Enrichment by Proton Analysis (PEPA): a One-Dimensional 1H-NMR Approach for 13C Stable Isotope Tracer Studies in Metabolomics
A novel metabolomics approach for NMR-based stable isotope tracer studies called PEPA is presented, and its performance validated using human cancer cells. PEPA detects the position of carbon label in isotopically enriched metabolites and quantifies fractional enrichment by indirect determination of...
2017-02-23 | MTBLS247 | MetaboLights
ProteomicsComparison of deep plasma proteome enrichment methods
Plasma is a complex biological fluid containing extracellular vesicles (EVs), residual platelets, and soluble proteins, all of which can serve as biomarkers for pathological conditions. While conventional plasma proteomics typically identifies hundreds of proteins, recent enrichment strategies have ...
ORGANISM(S): Homo sapiens (Human) 
2025-10-17 | PXD063673 | Pride
Enrichplus Proteograph Magnet Timstof Enrichment Plasma Evs Lc-ms/ms 
ProteomicsEvaluating Protein Enrichment Methods to Improve Biomarker Discovery in Equine Neurological Disorders
This study evaluated and compared three sample preparation strategies for proteomic analysis of equine cerebrospinal fluid (CSF): native in-solution digestion, the ProteoMiner™ Small-Capacity Enrichment Kit (Bio-Rad®), and the PreOmics Enrich-iST™ Kit (PreOmics®). CSF is in direct contact with the c...
ORGANISM(S): Equus caballus (Horse) 
2026-04-13 | PXD063239 | Pride
Cerebrospinal fluid (csf)  proteomics  native digestion  proteominer™ small-capacity enrichment kit  preomics enrich-ist™  liquid chromatography with tandem mass spectrometry (lc-ms/ms) 
ProteomicsComprehensive evaluation of different TiO2-based phosphopeptide enrichment and fractionation methods for phosphoproteomics
We performed a comprehensive evaluation of four different TiO2-based phosphopeptide enrichment methods using different non-phosphopeptide excluders. Then, two phosphopeptide fractionation methods, including the ammonia-based and the TEA based high pH reversed-phase fractionation, are also evaluated.
ORGANISM(S): Homo Sapiens 
2022-06-02 | PXD034264 |
ProteomicsComparison of affinity enrichment methods for O-GlcNAc proteomics
In this work, by using the lysates of PANC-1 (a pancreatic cancer cell line), we provided a head-to-head comparison of three affinity enrichment methods/materials (i.e., antibody, lectin AANL6, and an OGA mutant) for O-GlcNAc proteomics. The enrichment was performed before or after high-pH HPLC frac...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Saccharomyces Cerevisiae (ncbitaxon:4932) 
2024-04-23 | MSV000094604 | MassIVE
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