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MetabolomicsHierarchical Carbon Metabolism in Iron-Deficient Bacteria Favors Iron-Scavenging Strategy

High-affinity iron (Fe) scavenging compounds, or siderophores, are widely employed by soil bacteria to survive scarcity in bioavailable Fe. Siderophore biosynthesis relies on cellular carbon metabolism, despite reported decrease in both carbon uptake and Fe-containing metabolic proteins in Fe-def...

2020-12-05 | MTBLS1715 | MetaboLights
MetabolomicsMetabolomic Analysis of Candida albicans under Metal-based anti-fungal strategy

Based on a unique pathogen iron interplay mechanism, this work establishes a new therapeutic paradigm targeting iron homeostasis at the host-pathogen interface via sequential metabolic priming, offering a broadly applicable strategy to reprogram resource asymmetry and combat fungal infection.

2026-05-10 | MTBLS13715 | MetaboLights
ProteomicsEnrichment strategy for searching missing protein
Enrichment strategy for searching missing protein is a project under the guidance of Chromosome-Centric Human Proteome Project (C-HPP). We developed systematical enrichment strategies for revealing the existence of MPs including low molecular weight (LMW), membrane proteins, post-translational modif...
ORGANISM(S): Homo sapiens (Human) 
2015-07-14 | PXD002255 | Pride
Missing protein Chpp Enrichment strategy 
ProteomicsA Chemoselective Enrichment Strategy for In-Depth Coverage of the Methyllysine Proteome
There are the raw data of Kme1 peptides identification in the paper titled "A Chemoselective Enrichment Strategy for In-Depth Coverage of the Methyllysine Proteome".
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090) 
2024-07-28 | MSV000095469 | MassIVE
ProteomicsEffective Enrichment Strategy Using Boronic Acid Functionalized Mesoporous Graphene Silica Composites for Intact Glycopeptide Analysis in Human Serum
The heterogeneity and low abundance of protein glycosylation present challenging barriers to the analysis of intact glycopeptides, which is key to comprehensively understanding the role of glycosylation in an organism. Efficient and specific enrichment of intact glycopeptides could help greatly with...
ORGANISM(S): Human 
2021-04-28 | MSV000087324 | MassIVE
Genomicsenrichment culture
Amplicon sequencing of Nitrotoga enrichment cultures
PRJDB8089 | ENA
ProteomicsEnrichment strategy for searching missing protein
Enrichment strategy for searching missing protein is a project under the guidance of Chromosome-Centric Human Proteome Project (C-HPP). We developed systematical enrichment strategies for revealing the existence of MPs including low molecular weight (LMW), membrane proteins, post-translational modif...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-06-12 | MSV000083957 | MassIVE
ProteomicsCleavable Hydrophobic Derivatization Strategy for Enrichment and Identification of Phosphorylated Lysine Peptides
Herein, a cleavable hydrophobic derivatization (CHD) strategy was established for the enrichment and identification of pLys peptides. By CHD, 2,5-dioxopyrrolidin-1-yl-3-(decyldisulfanyl)propanoate (Dec-disulf-NHS) was synthesized to react with lysine dephosphorylated peptides, and then the derived p...
ORGANISM(S): Escherichia Coli 
2019-02-12 | PXD012682 |
ProteomicsSsimultaneous enrichment of cysteine-containing peptides and phosphopeptides using a novel Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in combination with TiO2 chromatography
Cysteine is a rare and conserved amino acid involved in most cellular functions. The thiol group of cysteine can be subjected to diverse oxidative modifications that regulate most physio-pathological states. Here, a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) was synthesized to selectively ...
ORGANISM(S): Homo sapiens (Human) 
2016-06-13 | PXD002704 | Pride
Cyspat  reversible cys modifications  redox chemistry  protein phosphorylation  tio2 enrichment  cross-talk  proteomics 
ProteomicsA Spin-Tip Enrichment Strategy for the Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues
Post-translational modifications (PTMs) change protein structures and functions. Methods for the simultaneous enrichment of multiple PTM types can maximize coverage in analyses. We present a protocol using dual-functional Ti(IV)-immobilized metal affinity chromatography followed by mass spectrometry...
ORGANISM(S): Homo sapiens (Human) 
2022-08-12 | PXD033065 | Pride
Post-translational modifications (ptms) Phosphopeptides Pancreas N-glycopeptides Dual-functional ti(iv)-immobilized metal affinity chromatography (imac) Electrostatic repulsion hydrophilic interaction chromatography (erlic) Simultaneous enrichment 
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