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ProteomicsSsimultaneous enrichment of cysteine-containing peptides and phosphopeptides using a novel Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in combination with TiO2 chromatography
Cysteine is a rare and conserved amino acid involved in most cellular functions. The thiol group of cysteine can be subjected to diverse oxidative modifications that regulate most physio-pathological states. Here, a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) was synthesized to selectively ...
ORGANISM(S): Homo sapiens (Human) 
2016-06-13 | PXD002704 | Pride
Cyspat  reversible cys modifications  redox chemistry  protein phosphorylation  tio2 enrichment  cross-talk  proteomics 
ProteomicsEnriching cysteine-containing peptides using a sulfhydryl-reactive alkylating reagent with a phosphonic acid group, and immobilized metal affinity chromatography
The reduction of disulfide bonds and their subsequent alkylation is commonplace in typical proteomics workflows. Here, we highlight a sulfhydryl-reactive alkylating reagent with a phosphonic acid group (iodoacetamido-LC-phosphonic acid, 6C-CysPAT) that facilitates the enrichment of cysteine-containi...
ORGANISM(S): Homo sapiens (Human) 
2023-12-28 | PXD038382 | Pride
Faims Tmtpro Cyspat Fe3+-nta Orbitrap eclipse Isobaric tagging 
ProteomicsAdaptation of CysPAT technique to characterize endogenous S-nitrosylation in RAW264.7 cells
The aim of the study was to adapt the CysPAT technique to efficiently identify and characterize endogenous S-nitrosylation in cells under physiological conditions of an experiment from a small amount of sample. Using a macrophage cell line RAW 264.7 we compared the efficacy of the method in the iden...
ORGANISM(S): Mus musculus (Mouse) 
2022-02-22 | PXD008182 | Pride
Post-translational modifications (ptms) Immune system S-nitrosylation Proteomics Macrophages 
ProteomicsCombined cysteine oxidation (C6-CPT/CysPAT) proteomics and phosphoproteomics of pervanadate-treated of HEK293T cells
HEK293T cells were treated with pervanadate for 0, 5 or 15 minutes to evaluate the effects on the phosphorylation and cysteine oxidation status across the proteome. Samples were labelled withTMT 18plex and C6-CPT/CysPAT labelling was used to allow the simultaneous enrichment of both the phosphoprote...
ORGANISM(S): Homo sapiens (Human) 
2026-06-22 | PXD059503 | Pride
Hek293t Cysteine C6-cyspat C6-cpt Redox Phosphoproteomics Pervanadate 
UnknownSimultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography.
Not available
S-EPMC5054350 | biostudies-literature
UnknownEnriching Cysteine-Containing Peptides Using a Sulfhydryl-Reactive Alkylating Reagent with a Phosphonic Acid Group and Immobilized Metal Affinity Chromatography.
Not available
S-EPMC10311885 | biostudies-literature
ProteomicsProteomic analysis of human PARK2 knockout iPSC-derived neurons
Mitochondrial dysfunction plays a major role in the pathogenesis of sporadic Parkinson’s disease (PD) and familial PD caused by mutations in the PARK2 gene. The protein, parkin, is vital for mitochondrial function, but the lack of key PD phenotypes in PARK2 knockout (KO) rodent models has hindered i...
ORGANISM(S): Homo sapiens (Human) 
2019-09-03 | PXD007871 | Pride
Lc-msms Qexactive-hf Parkinson's disease Human ips cells 
ProteomicsProteomic analysis of GBA iPSC-dopamine neurons
Heterozygous mutations in the glucocerebrosidase gene (GBA) are the strongest common genetic risk factors for Parkinson’s disease (PD) present in around 5-10% of PD patients, resulting in lower age of onset and exacerbating disease progression, including an increased risk of dementia. However, the e...
ORGANISM(S): Homo sapiens (Human) 
2023-03-04 | PXD026691 | Pride
Neuritogenesis/parkinson’s/proteomics/stem cells/glucocerebrosidase/post-translational modification 
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