Here we present LipidCreator, a software that fully supports targeted lipidomics assay development. LipidCreator offers a comprehensive framework to compute MS/MS fragment masses for over 60 lipid classes. LipidCreator provides all functionalities needed to define fragments, manage stable isotope...
Proteus mirabilis WGLW4 were cultured, supernatant was extracted using HLB-SPE and 80% MeOH/water and metabolome was analyzed with QExactive HF.
Negative mode, Double KO and wt
A CSF fractionation experiment was conducted, where trypsin digested 20 µg un-depleted CSF peptides were separated into eleven fractions and analysed using an QExactive HF, resulting in 1194 identified proteins.
Here we present LipidCreator, a software that fully supports targeted lipidomics assay development. LipidCreator offers a comprehensive framework to compute MS/MS fragment masses for over 60 lipid classes. LipidCreator provides all functionalities needed to define fragments, manage stable isotope...
The data contains 135 raw data produced from mass spectrometry (QExactive HF, Thermo Scientific), the bio-samples were sperm cells collected at nine sub-stages of mouse spermatogenesis. Proteins from each bio-samples were extracted and digested to peptides, then separated to five fractions and tripl...
Proteus mirabilis WGLW4 were cultured, supernatant was extracted using HLB-SPE and 80% MeOH/water and metabolome was analyzed with QExactive HF.
Negative mode, Double KO and wt
1. Sensitivity of FASP was tested using SDS lysates from HeLa cells and mouse brain. Peptides were analyzed using a QExactive HF-X instrument. We found that FASP allows effective processing samples at the low and sub-microgram range. 2. Whole cell lysates of Hela cells were processed with FASP using...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse)
Oncolytic virus therapy is a promising direction for cancer treatment. Numerous oncolytic virus strains are under development or examined in clinical trials, there are examples of FDA approved virus vaccines. Although evident progress exists, oncolytic viruses still suffer low efficiency and the mec...
Magnesium is essential for cellular life, but how it is homeostatically controlled still remains poorly understood. Here we report that members of CNNM family, which have been controversially implicated in both cellular Mg2+ influx and efflux, selectively bind to the TRPM7 channel to stimulate dival...
