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MetabolomicsLC-HRMS-Driven Computational Toolbox to Assess Extraction Protocols Dedicated to Untargeted Analysis: How to Ease Analyzing Pesticide-Contaminated Soils?
Metabolomics is a powerful approach that allows for high throughput analysis and the acquisition of large biochemical data. Nonetheless, it still faces several challenging requirements, such as the development of optimal extraction and analytical methods able to respond to its high qualitative and q...
2025-01-28 | MTBLS2044 | MetaboLights
MetabolomicsEvaluation of sample preparation protocols for quantitative NMR-based metabolomics

INTRODUCTION: Quantification of metabolites in biological fluids and tissues by NMR spectroscopy is challenged by the presence of abundant macromolecules and lipoproteins in samples, which give broad signals in the NMR spectra. To improve the quality of NMR spectra the different ...

2024-07-11 | MTBLS847 | MetaboLights
MetabolomicsEvaluation of Two Simultaneous Metabolomic and Proteomic Extraction Protocols Assessed by Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry
Not available
ORGANISM(S): Homo sapiens 
ST002391 | MetabolomicsWorkbench
MetabolomicsA robust intracellular metabolite extraction protocol for human neutrophil metabolic profiling
BACKGROUND: Neutrophils are phagocytic innate immune cells that play essential roles in host defence, but are also implicated in inflammatory diseases such as rheumatoid arthritis (RA) where they contribute to systemic inflammation and joint damage. Transcriptomic analysis of neutrophils has reveale...
2018-12-06 | MTBLS658 | MetaboLights
ProteomicsComparative Evaluation of Five Protocols for Protein Extraction from Stony Corals (Scleractinia) for Proteomics
Corals especially the reef-building species are very important to marine ecosystems. Proteomics has been used for researches on coral diseases, bleaching and responses to the environment change. Corals especially the reef-building species are very important to marine ecosystems. Proteomics has been ...
ORGANISM(S): Exaiptasia pallida Nematostella vectensis Chromera velia Acropora digitifera 
2022-07-24 | PXD006334 | Pride
Corals  protein extraction  proteomics  two dimensional electrophoresis (2-de)  maldi-tof-ms 
ProteomicsHistone datasets for evaluation of histone extraction protocols
Extracting histones from cells is a routine operation for studies that aim to characterize histones and their post-translational modifications (hPTMs). However, label-free quantitative mass spectrometry (MS) approaches, both data-dependent (DDA) and data-independent (DIA), require streamlined protoc...
ORGANISM(S): Homo sapiens (Human) 
2016-10-11 | PXD002885 | Pride
Extraction protocols Label-free ms Histone Targeted and untargeted analysis 
GenomicsAssessment of fecal DNA extraction protocols for metagenomic study
Assessment of fecal DNA extraction protocols for metagenomic study
PRJEB38036 | ENA
GenomicsDNA extraction protocols applied to waterbody in the Paris-Saclay region
DNA extraction protocols applied to waterbody in the Paris-Saclay region
PRJEB94317 | ENA
ProteomicsAssessment of protein extraction and digestion efficiency of well-established shotgun protocols for heart proteomics
In this study, we evaluated three well-established sample preparation methods for shotgun proteomics, named: i) UREA; ii) sodium dodecyl sulfate (SDS); and iii) filter-aided sample preparation (FASP) protocols. We focused our analysis on protein extraction and digestion from heart left ventricle (LV...
ORGANISM(S): Rattus norvegicus (Rat) 
2019-05-21 | PXD012591 | Pride
Lc-msms Rat Dimethyl labeling Heart Fasp 
MetabolomicsEvaluation of sample preparation methods for NMR-based metabolomics of cow milk
The quality of milk metabolome analyzed by nuclear magnetic resonance (NMR) is greatly influenced by the way samples are prepared. Although this analytical method is increasingly used to study milk metabolites, a thorough examination of available sample preparation protocols for milk has not been re...
2019-06-13 | MTBLS623 | MetaboLights
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