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ProteomicsPhosphorylation of the multi functional signal transducer B-cell adaptor protein (BCAP) facilitates recruitment of multiple SH2/SH3 proteins including Grb2.
B-cell adaptor protein (BCAP) is a multimodular, multi-functional signal transducer that regulates signal transduction processes in leukocytes including macrophages, B-cells and T-cells. In particular BCAP acts as a negative regulator of inflammatory signaling by the Toll-like receptors. Here we cha...
ORGANISM(S): Homo sapiens (Human) 
2019-09-16 | PXD014410 | Pride
Sh2 domains Toll-like receptors Phosphoinositide 3-kinase Human cell line hek293t bcap Phosphorylation Sh3 domains 
ProteomicsOrthogonal interactome profiling of RNF41
Ring finger protein 41 (RNF41) is an E3 ubiquitin ligase involved in the ubiquitination and degradation of many proteins such as ErbB3 receptors, BRUCE and parkin. Next to this, RNF41 regulates the intracellular trafficking of certain JAK-2 associated cytokine receptors by ubiquitinating and suppres...
ORGANISM(S): Homo sapiens (Human) 
2018-03-26 | PXD007794 | Pride
Human ap-ms viortrap bioid 
ProteomicsLoss-of-function of ubiquitin ligase adaptor LZTR1 triggers human disease by dysregulating RAS ubiquitination
Mutations in LZTR1, a substrate adaptor for cullin 3 (CUL3) ubiquitin ligase complexes1, have been recently associated with Noonan syndrome2,3 and familial Schwannomatosis4-6. Concordantly, we found that Lztr1+/- mice showed craniofacial abnormalities, cardio defects, and premature ageing; whereas l...
ORGANISM(S): Homo sapiens (Human) 
2018-11-04 | PXD007049 | Pride
Virotrap protein complex 
ProteomicsVirotrap: Abducting Protein Complexes from Mammalian Cells
Cell lysis is an inevitable step in classical affinity purification - mass spectrometry (AP-MS) strategies to map intracellular protein interactions. Cell homogenization implies a drastic change of the protein complex environment which strongly impedes comprehensive analysis. Complementary lysis con...
ORGANISM(S): Homo sapiens (Human) 
2016-04-27 | PXD000685 | Pride
Human vlp protein complex 
ProteomicsA well-controlled BioID design for endogenous bait proteins
The CRISPR/Cas9 revolution is profoundly changing the way life sciences technologies are used. Many assays now rely on engineered clonal cell lines to eliminate overexpression issues. Control cell lines are typically provided by non-engineered cells or by engineered clones implying a considerable ri...
ORGANISM(S): Homo sapiens (Human) 
2019-06-17 | PXD011702 | Pride
Protein protein interaction  bioid proximity labeling  (t2a) autocleavage  genome engineering  p53  base editor 
ProteomicsTargeting the lineage-specific long non-coding RNA LINC01212 as an effective anti-melanoma therapeutic strategy
Focal amplifications of 3p13-3p14 occur in about 10% of melanoma and are associated with poor prognosis. The melanoma-specific oncogene MITF resides at the epicenter of this amplicon1 . However, whether other loci present in this amplicon also contribute to melanomagenesis is unknown. Here we show t...
ORGANISM(S): Homo sapiens (Human) 
2016-01-13 | PXD002565 | Pride
Lncrna protein binding melanoma 
ProteomicsChIRP-MS of long non-coding RNA HOTAIR
Accumulating evidence highlights the role of long non-coding RNAs (lncRNA) in cellular homeostasis, and their dysregulation in disease settings. Most lncRNAs function by interacting with proteins or protein complexes. While several orthogonal methods have been developed to identify these proteins, e...
ORGANISM(S): Homo sapiens (Human) 
2022-02-15 | PXD029057 | Pride
Lncrna Hotair Chirp-ms 
ProteomicsLeveraging a self-cleaving peptide for tailored control in proximity labeling proteomics
PPIs play an important biological role in every aspect of cellular homeostasis and functioning. Proximity labeling mass spec-based proteomics overcomes challenges typically associated with other methods, and has quickly become the current state-of-the-art in the field. Nevertheless, tight control of...
ORGANISM(S): Homo sapiens (Human) 
2024-06-10 | PXD046412 | Pride
Bioid Sars-cov-2 nsp7 Turboid Proximity labeling Gr T2a Sars-cov-2 n 
ProteomicsLeveraging a self-cleaving peptide for tailored control in proximity labeling proteomics
Protein-protein interactions play an important biological role in every aspect of cellular homeostasis and functioning. Proximity labeling mass spectrometry-based proteomics overcomes challenges typically associated with other methods, and has quickly become the current state-of-the-art in the field...
ORGANISM(S): Homo sapiens (Human) 
2024-06-10 | PXD046413 | Pride
Bioid Sars-cov-2 nsp7 Turboid Proximity labeling Gr Sars-cov-2 n 
ProteomicsLeveraging a self-cleaving peptide for tailored control in proximity labeling proteomics
Protein-protein interactions play an important biological role in every aspect of cellular homeostasis and functioning. Proximity labeling mass spectrometry-based proteomics overcomes challenges typically associated with other methods, and has quickly become the current state-of-the-art in the field...
ORGANISM(S): Homo sapiens (Human) 
2024-06-10 | PXD046414 | Pride
Bioid Sars-cov-2 nsp7 Turboid Proximity labeling Gr Sars-cov-2 n 
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