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ProteomicsQuantitative Proteomic Identification of Proteins that are Responsive to RNF12 E3 Ligase Ubiquitin Activity
Rlim-/y mouse embryonic stem cells (mESCs) were transfected with either empty pCAGGS vector, HA-RNF12 or HA-RNF12 (H569A/C572A). Experiment was performed in biological triplicate (9 samples in total).
ORGANISM(S): Mus musculus (Mouse) 
2022-06-30 | PXD032317 | Pride
Mescs Rnf12 Tmt Lc-ms Quantification E3 ligase Ubiquitin 
ProteomicsAn ERK5-KLF2 signalling module regulates early embryonic gene expression dynamics and stem cell rejuvenation
CCE mESCs were transfected with either empty pCAGGS vector or HA-MEK5DD (S311D T315D) construct for 48 hours. 24 hours prior to lysis, mESCs were either treated with 10uM AX15836 or DMSO control, and transfected mESCs selected with puromycin. Conditions were performed in triplicate.
ORGANISM(S): Mus musculus (Mouse) 
2021-03-18 | PXD024679 | Pride
Erk5-klf2 Embryonic gene expression Tmt Stem cell rejuvenation Lc-ms Mass spectrometry Signalling 
ProteomicsThe FGF4-dependent phosphoproteome in mouse embryonic stem cells
Protein kinase signalling is a major mechanism by which embryonic stem cell pluripotency and differentiation is controlled. However, the pathways and components that regulate embryonic stem cell identity have not been systematically defined. Here, we employ FGF4 signalling as a model system to inves...
ORGANISM(S): Mus musculus (Mouse) 
2020-02-24 | PXD012069 | Pride
Kinase Phosphorylation Growth factor Mouse embryonic stem cell 
ProteomicsIdentification of the RNF12/RLIM proximal proteome
RNF12/RLIM is an E3 ubiquitin ligase that plays key developmental roles and is genetically disrupted in the neurodevelopmental disorder Tonne-Kalscheuer syndrome. RNF12/RLIM regulates X-chromosome inactivation and controls the expression of neural and gametogenesis related genes. To better understan...
ORGANISM(S): Mus musculus (Mouse) 
2024-01-09 | PXD046733 | Pride
E3 ubiquitin ligase  rnf12/rlim  embryonic stem cells  protein ubiquitylation  turboid chromatin  transcription 
ProteomicsA CDK1 phosphorylation site on Drosophila PAR-3 regulates neuroblast polarisation and sensory organ formation
The generation of distinct cell fates during development depends on asymmetric cell division of progenitor cells. In the central and peripheral nervous system of Drosophila, progenitor cells respectively called neuroblasts or sensory organ precursors use PAR polarity during mitosis to control cell f...
ORGANISM(S): Homo sapiens (Human) Drosophila melanogaster (Fruit fly) 
2024-07-02 | PXD051560 | Pride
Cell polarity Cell cycle Drosophila Asymmetric cell division 
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