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We developed a new method for the isolation of bronchial smooth muscle cells. This method is built upon a double fluorescent mouse SMA-GFP;NG2-DsRed and cell sorting. Using this method, we isolated bronchial smooth muscle cells from control and asthmatic C57BL/6J mice for gene profiling. Double fluo...
ORGANISM(S): Mus musculus 
Helicase Ded1 fine-tunes the reprogramming of gene expression conferred by inhibition of TORC1 by rapamycin.
We optimized an SDS depletion protocol involving KCl to precipitate the surfactant from intact proteins in the presence of urea at high pH. Provided is a comparison of 4 samples preciipitated at low pH (8) vs high pH (12), with (8 M) vs without (0 M) urea.
ORGANISM(S): Bos taurus (Bovine) 
2025-08-04 | PXD061512 | Pride
Custom oligonucleotide array CGH. We designed two fine-tiling oligonucleotide microarrays to cover the specific amplicons observed at chromosome 2p22.1 and 10q11.21 This was undertaken using the Agilent custom array design tool e-Array (Agilent, Santa Clara, CA, USA; https://earray.chem.agilent.com/...
ORGANISM(S): Homo sapiens 
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