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ProteomicsGetting more out of FLAG-Tag co-immunoprecipitation mass spectrometry experiments using FAIMS.
Co-immunoprecipitation of proteins coupled to mass spectrometry is critical for the understanding of protein interaction networks. In instances where a suitable antibody is not available, it is common to graft synthetic tags onto target protein sequences and allowing the use of commercially availabl...
ORGANISM(S): Homo sapiens (Human) 
2023-02-21 | PXD028965 | Pride
Faims Co-ip mass spectrometry Flag tag Proteomics Ion mobility 
ProteomicsImmunoprecipitation coupled to LC-MS/MS analysis of Flag and Flag-DJ-1 complexes in HEK293T cells
This dataset contains raw mass spectrometry data and associated protein and peptide identification results from anti-Flag immunoprecipitation coupled to LC-MS/MS analysis of Flag and Flag-DJ-1 complexes in HEK293T cells. The dataset was generated to identify candidate DJ-1-associated proteins and is...
ORGANISM(S): Homo sapiens (Human) 
2026-07-24 | PXD081552 | Pride
Affinity purification-mass spectrometry Hek293t cells Dj-1 Immunoprecipitation Park7 Protein-protein interactions Lc-ms/ms 
GenomicsRNA Immunoprecipitation(RIP)-seq of Flag-USP39 in ovarian cancer cells
RNA Immunoprecipitation(RIP)-seq of Flag-USP39 in ovarian cancer cells
PRJNA661211 | ENA
GenomicsChromatin Immunoprecipitation(ChIP)-seq of Flag-USP39 in ovarian cancer cells
Chromatin Immunoprecipitation(ChIP)-seq of Flag-USP39 in ovarian cancer cells
PRJNA661666 | ENA
GenomicsChromatin immunoprecipitation in mouse Epiblast stem cells induced with FLAG-Mixl1
Chromatin immunoprecipitation in mouse Epiblast stem cells induced with FLAG-Mixl1
PRJNA675789 | ENA
GenomicsNse4-FLAG CHIP-seq analysis in gcn5, ubp8, ada2 deletion background in S.pombe, control wt 501 - no tag
to find functional relevance between SMC5/6 complex and SAGA histone acetyltransferase complex in Schizosaccharomyces pombe by chromatin immunoprecipitation of Nse4-FLAG tagged protein from gcn5, ubp8 and ada2 deletion strains and 2 control strains (WT - Nse4-FLAG, 501 - no tag).
ORGANISM(S): Schizosaccharomyces pombe 
2023-02-20 | E-MTAB-12401 | biostudies-arrayexpress
TranscriptomicsFlag Chromatin IP in Cyclin D1 Flag-HA tagged E.14.5 embryos versus Flag Chromatin IP in wildtype E.14.5 embryos
Cyclin D1 belongs to the core cell cycle machinery1, and it is frequently overexpressed in human cancers2. The full repertoire of cyclin D1 functions in normal development and in cancer cells is currently unknown. To address this question, here we introduce a novel approach that allows one to deter...
ORGANISM(S): Mus musculus 
2010-05-15 | E-GEOD-13632 | biostudies-arrayexpress
ProteomicsLC-MSMS with isobaric tagging on Flag-coimmunoprecipitation eluates from Flag-Pgrmc1 and Y113F Flag-Pgrmc1 expressing Pgrmc1 KO mouse liver membranes
Progesterone receptor membrane component 1 (PGRMC1) is a heme binding protein implicated in a wide range of cellular functions. Our previous studies showed that PGRMC1 binds to cytochromes P450 in yeast and mammalian cells and promotes activity of these enzymes. Recently, the paralog PGRMC2 was show...
ORGANISM(S): Mus musculus (Mouse) 
2022-02-15 | PXD028284 | Pride
Lc-msms Mouse Tmt Co-immunoprecipitation Y113f flag-pgrmc1 Bound Membrane Pgrmc1 Flag-pgrmc1 Liver Eluate Isobaric tag 
ProteomicsFLAG-PHF3 interactome in HEK293 cells
FLAG-hPHF3 was transiently expressed in human HEK293 cells, followed by anti-FLAG immunoprecipitation, on-bead digest and NanoLC-MS analysis.
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD026292 | Pride
Human Anti-flag immunoprecipitation Nanolc-ms analysis On-bead digest 
ProteomicsFLAG-BMAL1 Immunoprecipitation followed by LC MS/MS in mESCs
Immunoprecipitation with anti-FLAG antibody in nuclear extracts of FLAG-BMAL1 expressing mESCs. LC MS/MS was used to analyze the interactome of BMAL1 in mESCs.
ORGANISM(S): Mus musculus (Mouse) 
2025-08-16 | PXD050769 | Pride
Bmal1  mescs 
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