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UnknownPersistent Directed Flag Laplacian (PDFL)-Based Machine Learning for Protein-Ligand Binding Affinity Prediction.
Not available
S-EPMC12020371 | biostudies-literature
ProteomicsAnti-FLAG-affinity purification of Umbrea associated factors
Anti-FLAG-affinity purification of Umbrea associated factors from nuclear extracts of Drosophila Schneider cells expressing FLAG-HA-Umbrea. Anti-FLAG -affinity purifications from Schneider cell nuclear extracts not expressing any FLAG-tagged protein served as a control SDS-PAGE separting both sa...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2013-10-03 | PXD000163 | Pride
Fruitfly Umbrea Drosophila melanogaster 
GenomicsmRNA sequencing of samples coming from Translating Ribosome Affinity Purification (TRAP) using MSH1P::FLAG::RPL18
mRNA sequencing of samples coming from Translating Ribosome Affinity Purification (TRAP) using MSH1P::FLAG::RPL18
PRJNA470540 | ENA
ProteomicsIdentification of host interactors of Brucella BT4E effector proteins by Flag-tag affinity purification coupled with LC-MS/MS in HEK293T cells
This study aimed to systematically identify host cellular proteins that interact with newly discovered Brucella abortus type IV secretion system (T4SS) effector proteins, designated BT4E3, BT4E4, BT4E19, BT4E32, BT4E33, BT4E37, and BT4E43. To achieve this, we employed an affinity purification–mass s...
ORGANISM(S): Homo sapiens (Human) 
2026-02-13 | PXD068987 | Pride
Brucella bt4e effector proteins Hek293t Lc-ms/ms Flag-tag affinity purification 
ProteomicsArabidopsis cell LC-MSMS - Proteomics analysis reveals a highly heterogeneous proteasome composition and the post-translational regulation of peptidase activity under pathogen signaling in plants
26S proteasome was affinity-purified with anti-FLAG beads and separated by 2-DE method. Each spot was cut and digested by Trypsin enzyme, which finaly applied for LC-MS/MS nalysis using a LTQ-Orbitrap XL (Thermo Scientific). The obtained spectra were compared against 35,386 sequences in The Arabidop...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2014-07-28 | PXD000417 | Pride
26sp 
ProteomicsThe Drosophila CoREST interactome
Affinity purification of dCoREST-interacting proteins from Drosophila melanogaster S2 cell nuclear extracts. An antibody recognizing both dCoREST isoforms was used to affinity purify proteins interacting with endogenous dCoREST from S2 nuclear extract. In addition, nuclear extracts from S2 cell line...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2019-11-11 | PXD014857 | Pride
Endogenous dcorest co-immunoprecipitation Flag-tagged dcorest-l co-immunoprecipitation Flag-tagged dcorest-m co-immunoprecipitation Dcorest proteome 
TranscriptomicsIdentity of root peridermis cells undergoing suberization in Arabidopsis
In order to unravel the molecular processes involved in the nature of the root peridermis tissue, as well as in the suberin biosynthesis pathways occurring in this tissue, we have made use of Translating Ribosome Affinity Purification (TRAP) followed by RNA sequencing (TRAP-SEQ) on two marker lines;...
ORGANISM(S): Arabidopsis thaliana 
2020-12-14 | E-MTAB-8949 | biostudies-arrayexpress
ProteomicsMudPIT analyses of FLAG-DYRK1A and FLAG-Controls affinity-purified from cytoplasmic extracts of HEK293 cells
Approximately 10E9 cells for each cell line (FLAG-DYRK1A, FLAG-DYRK1A-K188R, and FLAG-controls) were collected and washed with PBS. Cells were swollen for 15 minutes in hypertonic buffer (Buffer A: 10 mM Hepes pH 7.9, 1.5 mM MgCl2, 10 mM KCl, 0.5 mM DTT) freshly supplemented with protease inhibitor ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-07-23 | MSV000085815 | MassIVE
ProteomicsGenetic manipulation of Hmr leads to altered protein-protein interactions network
In this study we use Affinity Purification coupled with Mass Spectrometry (AP-MS) to test the effect of HMR overexpression (Hmr.over) or mutation (Hmr.dC and Hmr.2) on the interactome of HMR and in relation to the Speciation Core Complex (SCC). All affinity purifications were performed with a FLAG a...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2021-08-11 | PXD023193 | Pride
: chromatin  speciation  nucleolus  centromere  heterochromatin  chromocenter  drosophila  development  hybrids  hmr  bess  hmrdc  hmr2 
ProteomicsAffinity proteomics on A375 cells with N-Flag or N-Flag PTPN23 OE
The interacting proteins of PTPN23 on A375 cells were identified by affinity proteomics
ORGANISM(S): Homo sapiens (Human) 
2025-01-08 | PXD051577 | Pride
Affinity proteomics Ptpn23 Melanoma Human 
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