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TranscriptomicsFormalin Fixation at Low Temperature Better Preserves Nucleic Acid Integrity
INTRODUCTION. Fixation with formalin, a widely adopted procedure to preserve tissue samples, leads to extensive degradation of nucleic acids and thereby compromises procedures like microarray-based gene expression profiling. We hypothesized that RNA fragmentation is caused by activation of RNAses du...
ORGANISM(S): Homo sapiens 
2011-09-04 | E-GEOD-27175 | biostudies-arrayexpress
ProteomicsFormaldehyde fixation helps preserve the proteome state during single-cell proteomics sample processing and analysis
Mass spectrometry-based single-cell proteomics (SCP) is gaining momentum but remains limited to a few laboratories due to the high costs and specialized expertise required. The ability to send samples to specialized core facilities would benefit non-specialist labs and popularize SCP for biological ...
ORGANISM(S): Homo sapiens (Human) 
2026-02-09 | PXD056327 | Pride
Single-cell proteomics Formaldehyde fixation Facs One-tip 
ProteomicsFormaldehyde fixation helps preserving proteome state during single-cell proteomics sample processing and analysis
While mass spectrometry-based single cell proteomics (SCP) is gaining significant momentum, it is largely limited to a few laboratories worldwide. The ability to send samples to specialized core facilities, collaborators, would greatly benefit non-specialists laboratories or those unable to afford t...
ORGANISM(S): Homo sapiens (Human) 
2025-02-05 | PXD054445 | Pride
Single-cell proteomics Formaldehyde fixation 
TranscriptomicsThe global expression profile of Bacillus subtilis to the toxic carbonyls methylglyoxal (MG) and formaldehyde (FA)
Abstract of associated menuscript; Quinones and alpha,beta-unsaturated carbonyls are naturally occurring electrophiles that target cysteine residues via thiol-(S)-alkylation. We analyzed the global expression profile of Bacillus subtilis to the toxic carbonyls methylglyoxal (MG) and formaldehyde (FA...
ORGANISM(S): Bacillus subtilis 
2010-05-15 | E-GEOD-13925 | biostudies-arrayexpress
ProteomicsFixated rat liver tissue - Cleavable crosslinkers as tissue fixation reagents for proteomic analysis
Formaldehyde fixation is widely used for long-term maintenance of tissue. However, due to formaldehyde-induced cross-links, fixated tissue proteins are difficult to extract hampering the performance of mass spectrometry (MS)-proteomic analysis on formaldehyde-fixated tissue. Recent years saw the use...
ORGANISM(S): Rattus norvegicus (Rat) 
2018-01-29 | PXD002169 | Pride
Rat liver Tissue fixation Cleavable crosslinkers Reversible crosslinking 
TranscriptomicsAnalysis of RNA quantitation in formaldehyde-fixed cells
Fluorescence-activated cell sorting (FACS) is a sensitive and valuable technique to characterize cellular subpopulations and great advances have been made using this approach. Cells are often fixed with formaldehyde prior to the sorting process to preserve cell morphology and maintain the expression...
ORGANISM(S): Homo sapiens 
2013-09-09 | E-GEOD-50690 | biostudies-arrayexpress
ClinicalCarnoy’s Solution Versus Formalin in Rectal and Gastric Cancer Specimens Following: a Randomized Trial
This study evaluates the number of lymph nodes dissected in specimens following fixation with 10% neutral buffered formaldehyde or Carnoy’s solution. Specimens were randomized for fixation in each solution.
2204199 | ecrin-mdr-crc
GenomicsEnhancing single-cell ATAC sequencing with formaldehyde fixation, cryopreservation, and multiplexing for flexible analysis [ATAC-seq]
Enhancing single-cell ATAC sequencing with formaldehyde fixation, cryopreservation, and multiplexing for flexible analysis [ATAC-seq]
PRJNA1185742 | ENA
ProteomicsConventional cell fixation improves performance of in situ crosslinking mass spectrometry while preserving cellular ultrastructure
We developed an approach to in situ XL-MS which utilizes pre-stabilization with formaldehyde prior to crosslinker introduction. Applications of this method can boost crosslinker signal and allows for membrane-impermeable reagents to be used for studying protein-interaction landscapes in intact human...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2024-04-12 | PXD051075 | Pride
In situ xl-ms Formaldehyde fixaiton Crosslinking mass spectrometry 
ProteomicsNon-reversible tissue fixation by EDC carbodiimide allows for imaging extracellular vesicles in situ
Extracellular vesicles (EVs) are secreted nanosized particles with many biological functions and a broad range of pathological associations. A major technical limitation to understanding the role of EVs in normal and diseased specimens has been the inability to visualize the spatial localization ...
ORGANISM(S): Bos taurus (Bovine) 
2019-11-18 | PXD015234 | Pride
Imaging extracellular vesicles fixation tissue 
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