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ProteomicsOne-Tip enables comprehensive proteome coverage in minimal cells and single zygotes
We present One-Tip, a lossless proteomics methodology that seamlessly combines swift, one-pot sample preparation with narrow-window data-independent acquisition mass spectrometric analysis. With simplest sample processing, One-Tip reproducibly identifies > 9,000 proteins from ~1000 cells and ~ 6,000...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2023-09-15 | PXD044991 | Pride
Astral Proteomics Zygote 
ProteomicsOne-STAGE Tip Method for TMT based Proteomic Analysis of Minimal Amount of Cells
LC-MS-based profiling of proteomes with isobaric tag labeling from low-quantity biological and clinical samples, including needle-core biopsies and laser capture microdissection, has been challenging due to limited amount and sample loss during preparation. To address this problem, we developed OnM ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-04-18 | MSV000091734 | MassIVE
ProteomicsOne-step N-terminomics based on isolation of protein N-terminal peptides from LysargiNase digests by tip-based strong cation exchange chromatography
Large-scale isolation of protein N-terminal peptides from LysargiNase digests was achieved by one-step strong cation exchange chromatography with disposable and parallel-processable pipette-tip columns. Novel cleavage sites for signal or transit peptides and non-canonical translation initiation site...
ORGANISM(S): Homo Sapiens (human) 
2024-08-13 | PXD048917 | JPOST Repository
ProteomicsPho-Tip: one-pot dephosphorylation for rapid and sensitive analysis of DIA phosphoproteomics data
Here we introduce Pho-Tip, a low-loss one-pot dephosphorylation strategy. We show that Pho-Tip enables comprehensive mapping of phosphorylated peptide sequences, facilitating streamlined creation of experiment-focused in silico predicted spectral libraries and thus rapid and sensitive analysis of DI...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2026-02-17 | PXD070420 | Pride
Phosphorlyation Dia Human S. cerevisiae Yeast Hela High-throughput Phosphopeptides Quantitative proteomics 
UnknownOne-Tip enables comprehensive proteome coverage in minimal cells and single zygotes.
Not available
S-EPMC10951212 | biostudies-literature
UnknownHigh-throughput proteome integral solubility alteration assay for low cell input using One-Tip.
Not available
S-EPMC12474936 | biostudies-literature
ProteomicsIn-depth Phosphoproteome Analysis from Microscale Samples with Rapid In-Situ One-Tip IMAC Strategy
In this study, we developed a simple and rapid in-situ one-tip IMAC phosphoproteomics workflow integrated with data dependent acquisition without exclusion mass spectrometry for microscale phosphoproteomic analysis. The phosphopeptide enrichment material, made from hydrophilic cotton fibers with imm...
ORGANISM(S): Homo Sapiens 
2026-01-23 | PXD073439 |
ProteomicsUltrasensitive Phosphoproteome Analysis of Microscale Samples via Rapid In-Situ One-Tip IMAC Enrichment Strategy
In this study, we developed a novel phosphopeptide enrichment tip (Ti-PAN) based on highly hydrophilic textile PAN fibers, enabling rapid in-situ One-Tip IMAC enrichment within 8 minutes. Using this approach, we identified 2,139 to 17,546 phosphosites across 645 to 3,531 phosphoproteins from samples...
ORGANISM(S): Homo Sapiens Mus Musculus 
2025-11-25 | PXD071174 |
UnknownOne-step N-Terminomics Based on Isolation of Protein N-Terminal Peptides From LysargiNase Digests by Tip-Based Strong Cation Exchange Chromatography.
Not available
S-EPMC11382313 | biostudies-literature
TranscriptomicsEndothelin2 signaling in the neural retina promotes the endothelial tip cell state and inhibits angiogenesis
Endothelin signaling is required for neural crest migration and homeostatic regulation of blood pressure. Here we report that constitutive over-expression of Endothelin-2 (Edn2) in the mouse retina perturbs vascular development by inhibiting endothelial cell (EC) migration across the retinal surfac...
ORGANISM(S): Mus musculus 
2014-01-01 | E-GEOD-50059 | biostudies-arrayexpress
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