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ProteomicsA genetically-encoded crosslinker screen identifies SERBP1 as a PKC substrate influencing translation and cell division
The PKC-regulated genome protective pathway provides transformed cells a failsafe to successfully complete mitosis. Despite the necessary role for Aurora B in this programme, it is unclear whether its requirement is sufficient or if other PKC cell cycle targets are involved. To address this, we deve...
ORGANISM(S): Homo sapiens (Human) 
2021-12-09 | PXD029091 | Pride
Human 
ProteomicsIdentification of a TOR-regulated phosphoproteome and the regulation of cellular protein synthesis
We describe an in depth analysis in fission yeast of changes in the phosphoproteome after inhibition of the TOR protein kinases. A total of 756 phosphosites on 316 proteins showed significant and rapid changes in phosphorylation levels after TOR kinase inhibition when the rate of protein synthesis r...
ORGANISM(S): Schizosaccharomyces pombe 972h- 
2021-07-09 | PXD023664 | Pride
Phosphoproteomics Fission yeast Tmt S. pombe Tor 
UnknownA mathematical framework for the emergence of winners and losers in cell competition.
Not available
S-EPMC7618079 | biostudies-literature
ProteomicsStructure of the Cohesin Loader Scc2-Scc4 and its Binding to a Folded Cohesin
The functions of cohesin are central to genome integrity, chromosome organization, and transcription regulation through its prevention of premature sister-chromatid separation and the formation of DNA loops. The loading of cohesin onto chromatin depends on the Scc2-Scc4 complex, however, little is k...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2017-01-11 | PXD004692 | Pride
Cohesin Cross-linking Cx-ms 
TranscriptomicsGene expression analysis of Neoechinulin B (NeoB)-treated Huh7.5.1 cells
Huh-7.5.1 cells were treated with 0.2% DMSO, 20 microM NeoB for 24 h. Treatment with 0.2% DMSO for 24h was prepared as non-treated Huh7.5.1 cells. Huh7.5.1 cells were kindly provided by Prof. Francis Chisari at The Scripps Research Institute. Total RNA obtained from NeoB-treated and un-treated Huh7...
ORGANISM(S): Homo sapiens 
2015-08-31 | E-GEOD-63026 | biostudies-arrayexpress
ProteomicsUSP7 is a tumor-specific WNT activator for APC-mutated colorectal cancer by mediating B-catenin deu
The tumor suppressor gene adenomatous polyposis coli (APC) is mutated in most colorectal cancers (CRC) resulting in constitutive Wnt activation. To understand the Wnt-activating mechanism of APC mutation, we applied CRISPR/Cas9 technology to engineer various APC-truncated isogenic lines. We find tha...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2017-11-09 | PXD007947 | Pride
Apc Lfq 
ProteomicsFunctional crosstalk between the cohesin loader and chromatin remodelers
The cohesin complex has crucial roles in many structural and functional aspects of chromosomes including sister chromatid cohesion, genome organization, gene transcription and DNA repair. Cohesin recruitment onto chromosomes requires nucleosome free DNA and a specialized cohesin loader complex compr...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-11-29 | PXD033446 | Pride
Chromatin Cross-linking 
ProteomicsCyclin-dependant kinase (CDK) orders the cell cycle via substrate specific thresholds
The appropriate ordering of the cell cycle events in eukaryoates is thought to be brought about by qualitative differences in the substrate specificity of multiple differentially expression Cyclin-CDK complexes. Our analysis of fission yeast supports an alternative quantitative model. Here we report...
ORGANISM(S): Schizosaccharomyces pombe 
2018-04-11 | PXD003598 | Pride
Mass spectrometry Cdk Silac Cell cycle S. pombe 
ProteomicsRedundancy and Specialisation of Cyclin Dependent Kinases
Cyclin dependent kinases (CDKs) lie at the heart of eukaryotic cell division, with different CDK complexes initiating DNA replication (S-CDKs) and mitosis (M-CDKs)1-3. However, the principles on which cyclin-CDKs organise the cell cycle are still contentious, with current theories suggesting that ei...
ORGANISM(S): Schizosaccharomyces pombe 972h- 
2022-03-22 | PXD029073 | Pride
Phosphoproteomics Fission yeast Tmt S. pombe 
ProteomicsPP21Cdc55 phosphatase imposes phosphothreonine-specific ordering of cell cycle phosphorylation
In the quantitative model for cell cycle control, progression from G1 through S phase and into mitosis is ordered by thresholds of increasing cyclin-dependent kinase (Cdk) activity. How such thresholds are read out by substrates, that respond with the correct phosphorylation timing, if not known. In...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2017-01-23 | PXD004461 | Pride
Cdk Phosphatase Phosphorylation Silac Cell cycle 
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