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ProteomicsProteome and metabolome alterations in radish (Raphanus sativus L.) seedlings induced by inoculation with Agrobacterium tumefaciens
Here we provide a comprehensive explorative proteomics survey aiming at intensive charac-terization of metabolic shifts and molecular events accompanying plant responses to inoculation with A. tumefaciens culture. Based on the comprehensive proteomics dataset complemented with an untargeted metaboli...
ORGANISM(S): Raphanus sativus 
2025-05-07 | PXD060463 | Pride
Radish Shotgun proteomics Agrobacterium tumefaciens Metabolic shifts Crown gall tumor Agrobacterium Raphanus sativus Agrobacterial infection 
ProteomicsAdvanced glycation end products as markers of ageing in plants: glycation hotspots in the plant proteome
Glycation is a post-translational modification underlied by the interaction of protein amino and guanidino groups with carbonyl compounds like reducing sugars and α-dicarbonyls. In the first steps of this process, the protein amino groups react with reducing carbohydrates yielding the corresponding ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2017-06-19 | PXD006434 | Pride
Ages Plant proteomics Glycation 
ProteomicsValidation of filter aided sample preparation (FASP) approach for quantitative plant shotgun proteomics
Gel-free LC-based shotgun proteomics represents the current gold standard of proteome analysis due to its outstanding throughput, analytical resolution and reproducibility. Thereby, the efficiency of sample preparation, i.e., protein isolation, solubilization and proteolysis, directly affects the co...
ORGANISM(S): Bos taurus (Bovine) Pisum sativum (Garden pea) Arabidopsis thaliana (Mouse-ear cress) Homo sapiens (Human) 
2023-03-11 | PXD025897 | Pride
Plant proteomics Filter aided sample preparation (fasp) Shotgun proteomics Lc-ms Phenol extraction Label-free quantification Detergent-assisted proteolysis Sodium dodecyl sulfate 
ProteomicsResponsivity of two pea genotypes to the symbiosis with rhizobia and arbuscular mycorrhiza fungi – a proteomics aspect of the “Efficiency of Interactions with Beneficial Soil Microoganisms” trait
It is well-known that individual pea (Pisum sativum L.) cultivars differ in their symbiotic responsivity. This trait is typically manifested with an increase in seed weights due to inoculation with rhizobial bacteria and arbuscular mycorrhizal fungi. The aim of this work was to characterize the alte...
ORGANISM(S): Pisum sativum (Garden pea) 
2025-02-24 | PXD058701 | Pride
Proteomics Legume-rhizobial symbiosis Arbuscular mycorrhizal Pea Efficiency of interactions with beneficial soil microorganisms 
ProteomicsSeed proteome of pea lines with different responsivity to simultaneous inoculation with arbuscular mycorrhiza fungi and rhizobia
Legume crops represent the major source of food protein and contribute to human nutrition and animal feeding. An essential improvement of their productivity can be achieved by symbiosis with beneficial soil microorganisms – rhizobia (Rh) and arbuscular mycorrhizal (AM) fungi. The efficiency of these...
ORGANISM(S): Pisum sativum (Garden pea) 
2019-05-03 | PXD013479 | Pride
Arbuscular mycorrhiza  beneficial soil microorganisms  combined inoculation  inoculation efficiency  lc-ms  pea (pisum sativum l.)  proteomics  rhizobial symbiosis  seed proteome 
ProteomicsProteomic analysis of the role of RALF34 small signaling peptides in the regulation of cucumber (Cucumis sativus) root development
To elucidate the functional role of the small signaling peptide RALF34 in the parental root meristem and its role in lateral root initiation, we studied changes in Cucumis sativus proteome during CsRALF34 gene overexpression. Composite plants with transgenic root systems carrying the construct for C...
ORGANISM(S): Cucumis sativus 
2023-05-10 | PXD037725 | Pride
Lateral root initiation Csralf34 Ralf34 Cucumber Proteome Cucumis sativus 
ProteomicsUHPLC-MS/MS analysis of S-nitrosylated proteins involved in autophagy in Triticum aestivum roots
In the present work, we analyzed the patterns of protein S-nitrosylation during the inductionof autophagy in сommon wheat (Triticum aestivum) roots. S-nitrosylated proteins were accumulated in plant tissues as a result of artificially induced autophagy, visualized usingWestern blot and identified by...
ORGANISM(S): Triticum aestivum (Wheat) 
2023-12-31 | PXD044484 | Pride
Autophagy Nitric oxide S-nitrosylation of proteins Triticum aestivum 
ProteomicsMetabolic responses to the zinc stress in the roots and leaves of Amaranthus caudatus: the proteomics view
Here we employ LC-MS-based bottom-up proteomics to address the metabolic responses of the A. caudatus root and leaves to moderate Zn stress. Thereby, we aimed identification of the Zn-responsive proteins in the roots, young and mature leaves of the A. caudatus plants exposed to Zn stress for seven d...
ORGANISM(S): Amaranthus caudatus 
2025-11-03 | PXD069557 | Pride
Amaranthus caudatus  zinc stress  bottom-up proteomics  metabolic adjustment 
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