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ProteomicsPulseDIA: data independent acquisition mass spectrometry using pulsed multi-injection gas phase frac-tionation
Here we present an enhanced gas phase fractionation (GPF)-assisted DIA-MS acquisition method called PulseDIA, which improves the specificity and sensitivity of Orbitrap-based DIA analysis.
ORGANISM(S): Homo Sapiens Escherichia Coli Mus Musculus 
PXD019379 |
ProteomicsImproved detection of tryptic immunoglobulin variable region peptides by chromatographic and gas-phase fractionation techniques.
Circulating antibodies provide valuable information about an individual’s immune status and constitute a significant portion of the human plasma proteome. Proteomic analysis of plasma is challenged by the large dynamic concentration range of plasma proteins, including antibodies. Peptide fractionati...
ORGANISM(S): Homo sapiens (Human) 
2024-08-09 | PXD046072 | Pride
Peptide fractionation Antibody Mass spectrometry Immune repertoire Multi-dimensional chromatography Ion-mobility Bottom-up proteomics De novo sequencing Plasma proteomics 
ProteomicsA combined gas phase separation strategy for ADP-ribosylated peptides
ADP-ribosylation (ADPr) is a posttranslational modification that is best studied using mass spectrometry. Method developments that are permissive to low inputs or baseline levels of protein ribosylation represent the next frontier in the field. High-field asymmetric waveform ion mobility spectrometr...
ORGANISM(S): Mus musculus (Mouse) 
2023-08-30 | PXD040898 | Pride
Mono-adp-ribose Gas-phase segmentation Ribosylation High field asymmetric waveform ion mobility spectrometry Gas-phase fragmentation 
ProteomicsFAIMS-GPF XL-MS: crosslinking-mass spectrometry based on gas-phase fractionation
Protein-protein interactions (PPIs) underpin nearly all cellular processes; therefore, mapping PPI and protein-protein association networks is critical for understanding how biological systems function in health and disease. However, many functionally relevant PPIs are transient and mediated by weak...
ORGANISM(S): Homo sapiens (Human) 
2026-05-27 | PXD064228 | Pride
Faims Cross-linking mass spectrometry Structural biology Interactomics 
ProteomicsAdvanced mass spectrometry workflows for accurate quantification of trace-level host cell proteins in drug products: benefits of FAIMS separation and gas-phase fractionation DIA
In this study, we investigated the benefits of adding high-field asymmetric ion mobility spectrometry (FAIMS) separation prior to data dependent acquisition (DDA) and gas phase fractionation (GPF) prior to data independent acquisition (DIA) LC-MS/MS analysis. Native digestion followed by LC-MS/MS wi...
ORGANISM(S): Mus musculus (Mouse) Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2023-05-07 | PXD039585 | Pride
Faims Data-dependent analysis (dda) Host cell proteins (hcp) Final drug products (dp) 
ProteomicsAdvanced mass spectrometry workflows for accurate quantification of trace-level host cell proteins in drug products: benefits of FAIMS separation and gas-phase fractionation DIA
In this study, we investigated the benefits of adding high-field asymmetric ion mobility spectrometry (FAIMS) separation prior to data dependent acquisition (DDA) and gas phase fractionation (GPF) prior to data independent acquisition (DIA) LC-MS/MS analysis. Native digestion followed by LC-MS/MS wi...
ORGANISM(S): Mus musculus (Mouse) Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2023-05-07 | PXD039582 | Pride
Data-independent analysis (dia) Host cell proteins (hcp) Final drug products (dp) 
ProteomicsComparing peptide identifications by FAIMS versus quadrupole gas phase fractionation
Gas phase fractionation techniques have become popular in the field of mass spectrometry to improve results without using more sample. High field asymmetric waveform ion mobility spectrometry (FAIMS) coupled to liquid chromatography-mass spectrometry (LC-MS) has been shown to increase peptide and pr...
ORGANISM(S): Homo Sapiens 
2023-07-03 | PXD043458 | panorama
UnknownEnhanced Lipidome Coverage in Shotgun Analyses by using Gas-Phase Fractionation.
Not available
S-EPMC5061616 | biostudies-literature
ProteomicsComparison of fractionation proteomics for local SWATH library building
For data-independent acquisition by means of sequential window acquisition of all theoretical fragment ion spectra (SWATH), a reference library of data-dependent acquisition (DDA) runs is typically used to correlate the quantitative data from the fragment ion spectra with peptide identifications. Th...
ORGANISM(S): Homo sapiens (Human) 
2017-07-04 | PXD006190 | Pride
Library building Comparative study Swath-ms Fractionation proteomics Hesc 
UnknownGas-phase fractionation DDA promotes in-depth DIA phosphoproteome analysis.
Not available
S-EPMC11787513 | biostudies-literature
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