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ProteomicsCBB staining showed beneficial effects on proteomic analysis employing PAGE separation followed with whole-gel slicing, in-gel digestion and quantitative LC-MS/MS
Proteomic analysis by combining PAGE separation, gel slicing and slice-by-slice LC-MS/MS has been frequently reported in recent years. Since the MS analysis would provide identities and quantities of the proteins along the whole lane, visualization by dye staining could be skipped to save time and ...
ORGANISM(S): Rattus norvegicus (Rat) 
2019-02-16 | PXD011689 | Pride
Sds-page In-gel digestion Proteomic analysis Cbb r-250 staining Whole gel slicing Quantitative lc-ms/ms Nondenaturing page 
MetabolomicsA Metabonomic Comparison of Human Serum and Plasma Subtypes Using Untargeted NMR Spectroscopy and UPLC-MS (UPLC-MS assays)
INTRODUCTION: Differences in the metabolite profiles between serum and plasma are incompletely understood. OBJECTIVES: To evaluate metabolic profile differences between serum and plasma and among plasma sample subtypes. METHODS: We analyzed serum, platelet rich plasma (PRP), platelet poo...
2018-07-25 | MTBLS465 | MetaboLights
MetabolomicsA Metabonomic Comparison of Human Serum and Plasma Subtypes Using Untargeted NMR Spectroscopy and UPLC-MS (NMR assays)
INTRODUCTION: Differences in the metabolite profiles between serum and plasma are incompletely understood. OBJECTIVES: To evaluate metabolic profile differences between serum and plasma and among plasma sample subtypes. METHODS: We analyzed serum, platelet rich plasma (PRP), platelet poo...
2018-07-25 | MTBLS470 | MetaboLights
ProteomicsGel-free/label-free proteomic, biochemical and photosynthetic anaysis of cowpea resistance against Cowpea severe mosaic virus
In this present work, photosynthetic and biochemical parameters in conjunction with gel-free/label-free proteomic analysis were assessed in a resistant cowpea genotype challenged with CPSMV and compared with the corresponding data of the mock-inoculated control as an attempt to find out some clues t...
ORGANISM(S): Vigna unguiculata 
2024-09-12 | PXD005906 | Pride
Cowpea Incompatible interaction Physiology Biochemistry Gel-free/label-free proteomic 
ProteomicsAn alliance of gel-based and gel-free proteomic techniques displays substantial insight into the proteome of a virulent and an attenuated Histomonas meleagridis strain
The current work aimed to detect and identify significantly differentially expressed proteins between a virulent and an attenuated Histomonas meleagridis strain which were in vitro co-cultivated with Escherichia coli DH5alpha. This was done by applying 2D-DIGE and sequential window acquisition of al...
ORGANISM(S): Histomonas meleagridis 
2018-11-21 | PXD009319 | Pride
Attenuation. Protozoa Swath ms Histomonas meleagridis Virulence factors Comparative proteomic analysis 2d-dige Maldi-tof/tof Lc-ms/ms 
ProteomicsAn alliance of gel-based and gel-free proteomic techniques displays substantial insight into the proteome of a virulent and an attenuated Histomonas meleagridis strain
The current work aimed to detect and identify significant differentially expressed proteins between a virulent and an attenuated Histomonas meleagridis strain which was in vitro co-cultivated with Escherichia coli DH5alpha. This was done by applying 2D-DIGE and sequential window acquisition of all t...
ORGANISM(S): Histomonas meleagridis 
2018-11-22 | PXD009332 | Pride
Attenuation. Protozoa Swath ms Histomonas meleagridis Virulence factors Comparative proteomic analysis 2d-dige Maldi-tof/tof Lc-ms/ms 
ProteomicsAn alliance of gel-based and gel-free proteomic techniques displays substantial insight into the proteome of a virulent and an attenuated Histomonas meleagridis strain
The current work aimed to detect and identify significant differentially expressed proteins between a virulent and an attenuated Histomonas meleagridis strain which were in vitro co-cultivated with Escherichia coli DH5alpha. This was done by applying 2D-DIGE and sequential window acquisition of all ...
ORGANISM(S): Histomonas meleagridis 
2018-12-10 | PXD009316 | Pride
Attenuation. Protozoa Swath ms Histomonas meleagridis Virulence factors Comparative proteomic analysis 2d-dige Maldi-tof/tof Lc-ms/ms 
ProteomicsProtein Metabolism Underlying Heat Tolerance in Contrasting Creeping Bentgass Lines: Insights From Gel-Free Proteomics
One of the major disfunctions that occurs in heat-stressed plants is enhanced protein damage and a consequent decline in cellular protein content. Creeping bentgrass (Agrositis stolonifera L.) is an economically important perennial grass species which is largely used on high value turf areas but exp...
ORGANISM(S): Agrostis stolonifera 
2025-10-20 | PXD060809 | Pride
Heat stress  creeping bentgrass  polyubiquitin-omics  gel-free proteomics  proteostasis 
ProteomicsTube-gel digestion: a fast and reproducible sample preparation protocol for label-free quantitative proteomics of complex biological samples
Comparison of tube-gel protocol to stacking gel and liquid digestion for label-free extracted ion-current quantification of complex biological samples Dataset with known fold-changes (UPS1 spiked in yeast background at 25-10-5-2.5-1-0.5 fmol)
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2016-10-18 | PXD003841 | Pride
Label-free quantitative proteomics Liquid digestion Sample preparation Tube-gel Stacking gel 
ProteomicsGlobal mapping of rat plasma proteins with a native proteomic approach using nondenaturing micro 2DE and label-free quantitative LC-MS/MS
Plasma samples from adult male rats were separated by nondenaturing micro 2DE and a representative gel was selected, on which a total of 136 CBB-stained spots were numbered and subjected to in-gel digestion and label-free quantitative LC-MSE (MS/MS in data-independent acquisition mode). The analysi...
ORGANISM(S): Rattus norvegicus (Rat) 
2016-10-14 | PXD004395 | Pride
Native proteomics Adult male rat plasma proteome Nondenaturing micro 2de Global mapping Label-free quantitative lc-mse 
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