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ProteomicsLC-MS/MS-Based Proteomic Analysis of a Single Human-Derived Gel Band
LC-MS/MS-Based Proteomic Analysis of a Single Human-Derived Gel Band
ORGANISM(S): Homo sapiens (Human) 
2026-06-11 | PXD074137 | Pride
Xb10683gi 
ProteomicsAn alliance of gel-based and gel-free proteomic techniques displays substantial insight into the proteome of a virulent and an attenuated Histomonas meleagridis strain
The current work aimed to detect and identify significant differentially expressed proteins between a virulent and an attenuated Histomonas meleagridis strain which was in vitro co-cultivated with Escherichia coli DH5alpha. This was done by applying 2D-DIGE and sequential window acquisition of all t...
ORGANISM(S): Histomonas meleagridis 
2018-11-22 | PXD009332 | Pride
Attenuation. Protozoa Swath ms Histomonas meleagridis Virulence factors Comparative proteomic analysis 2d-dige Maldi-tof/tof Lc-ms/ms 
ProteomicsAn alliance of gel-based and gel-free proteomic techniques displays substantial insight into the proteome of a virulent and an attenuated Histomonas meleagridis strain
The current work aimed to detect and identify significant differentially expressed proteins between a virulent and an attenuated Histomonas meleagridis strain which were in vitro co-cultivated with Escherichia coli DH5alpha. This was done by applying 2D-DIGE and sequential window acquisition of all ...
ORGANISM(S): Histomonas meleagridis 
2018-12-10 | PXD009316 | Pride
Attenuation. Protozoa Swath ms Histomonas meleagridis Virulence factors Comparative proteomic analysis 2d-dige Maldi-tof/tof Lc-ms/ms 
ProteomicsGel based and gel-free search for plasma membrane proteins in chickpea (Cicer arietinum L.) augments the comprehensive data sets of membrane protein repertoire
Plasma membrane (PM) encompasses total cellular contents, serves as a living barrier regulates all cellular exchanges in a spatiotemporal fashion. Most of the essential tasks of PMs including molecular transport, cell-cell interaction and signal transduction are carried out by their proteinaceous co...
ORGANISM(S): Cicer arietinum (Chickpea) (Garbanzo) 
2024-12-05 | PXD003697 | Pride
Shotgun proteomics Plant proteomics High resolution mass spectrometry 
ProteomicsA protocol for global quantification of phosphoproteins combining metabolic labeling and gel-based proteomics
Differential proteomics targeting the abundance level and changes of proteins is commonly used in biology to determine changes induced by different physiological states in living organisms. Here, current methods comprise both label-free and label-based proteomics approaches. More specifically, besid...
ORGANISM(S): Bacillus pumilus 
2017-10-02 | PXD005262 | Pride
Peroxide stress adaptation Gel-based proteomics Phosphorylation 14n/15n metabolic labeling Bacillus pumilus Quantification 
ProteomicsAnalysis of mouse hippocampus membrane proteome by gel-based LC/MS
Mouse hippocampus membrane fractions were prepared using sucrose density gradient ultracentrifugation. Membrane proteins were solubilized using three different condition; 6-ACA/Triton X-100 or ProteoExtract Native Membrane Protein Extraction Kit (Calbiochem, Cat. No. 444810) or ProteoExtract Transme...
ORGANISM(S): Mus musculus (Mouse) 
2014-08-15 | PXD000311 | Pride
Mouse hippocampus Ltq orbitrap velos pro Membrane proteins Gel-based proteomics In-gel chymotrypsin/trypsin digestion 
OtherGel-based mass spectrometric analysis of hippocampal transmembrane proteins using high resolution LTQ Orbitrap Velos Pro.
Data from ProteomeXchange, PXD ID: PXD000311. Enzyme: trypsin, file: F017572.dat-ACA-SDS_T3.mzml. Published as part of Proteomics. 2014 Jul 10 . From the Abstract: {{i}} ... Proteins were extracted from pooled whole mouse brains, enriched membrane fractions were prepared using either two commercial...
ORGANISM(S): Mus_musculus_viruses, Mouse 
GPM32310002988 | GPMDB
Resource Reanalysis 
ProteomicsAn alliance of gel-based and gel-free proteomic techniques displays substantial insight into the proteome of a virulent and an attenuated Histomonas meleagridis strain
The current work aimed to detect and identify significantly differentially expressed proteins between a virulent and an attenuated Histomonas meleagridis strain which were in vitro co-cultivated with Escherichia coli DH5alpha. This was done by applying 2D-DIGE and sequential window acquisition of al...
ORGANISM(S): Histomonas meleagridis 
2018-11-21 | PXD009319 | Pride
Attenuation. Protozoa Swath ms Histomonas meleagridis Virulence factors Comparative proteomic analysis 2d-dige Maldi-tof/tof Lc-ms/ms 
ProteomicsPEPPI-MS- and GELFrEE-fractionated E. coli MG1655 whole cell lysate
Supplemental Files accompanying manuscript “PEPPI-MS: polyacrylamide gel-based prefractionation for analysis of intact proteoforms and protein complexes by mass spectrometry”.
ORGANISM(S): Escherichia Coli Str. K-12 Substr. Mg1655 (ncbitaxon:511145) 
2020-04-08 | MSV000085258 | MassIVE
ProteomicsGel based extracellular matrix proteome of jute
Extracellular matrix (ECM) is the first line of defense which is an inimitable organelle that perceives biotic and abiotic stresses and reprograms biological processes of host. It also activates innate immune responses in temporal and spatial manner and acts as physical scaffold that prevents the en...
ORGANISM(S): Corchorus olitorius 
2024-05-21 | PXD046939 | Pride
1-de sds page Mass spectrometry Jute Extracellular matrix 
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