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ProteomicsA protocol for global quantification of phosphoproteins combining metabolic labeling and gel-based proteomics
Differential proteomics targeting the abundance level and changes of proteins is commonly used in biology to determine changes induced by different physiological states in living organisms. Here, current methods comprise both label-free and label-based proteomics approaches. More specifically, besid...
ORGANISM(S): Bacillus pumilus 
2017-10-02 | PXD005262 | Pride
Peroxide stress adaptation Gel-based proteomics Phosphorylation 14n/15n metabolic labeling Bacillus pumilus Quantification 
ProteomicsWheat Allergens MALDI-TOF/TOF - Using 7 cm immobilized pH gradient strips to determine levels of clinically relevant proteins in wheat grain extracts
The aim of the work was to test relatively simple proteomics approach based on phenol extraction and two-dimensional gel electrophoresis (2-DE) with 7cm immobilized pH gradient strips for the determination of clinically relevant proteins in wheat grain. Using this approach, 157 2-DE spots were quan...
ORGANISM(S): Triticum aestivum (Wheat) 
2017-08-23 | PXD002067 | Pride
Grain Maldi-tof/tof Allergen Gel-based quantification Triticum aestivum 
ProteomicsAn alliance of gel-based and gel-free proteomic techniques displays substantial insight into the proteome of a virulent and an attenuated Histomonas meleagridis strain
The current work aimed to detect and identify significantly differentially expressed proteins between a virulent and an attenuated Histomonas meleagridis strain which were in vitro co-cultivated with Escherichia coli DH5alpha. This was done by applying 2D-DIGE and sequential window acquisition of al...
ORGANISM(S): Histomonas meleagridis 
2018-11-21 | PXD009319 | Pride
Attenuation. Protozoa Swath ms Histomonas meleagridis Virulence factors Comparative proteomic analysis 2d-dige Maldi-tof/tof Lc-ms/ms 
UnknownSmartphone-Based in-Gel Loop-Mediated Isothermal Amplification (gLAMP) System Enables Rapid Coliphage MS2 Quantification in Environmental Waters.
Not available
S-EPMC5990930 | biostudies-literature
ProteomicsA metabolic labeling strategy for relative protein quantification in Clostridioides difficile
Clostridioides difficile (formerly Clostridium difficile) is a Gram-positive, spore-forming pathogen which cases drug-induced Clostridioides difficile-associated diseases in hospitals worldwide. A detailed analysis of the proteome may provide new targets for drug development or therapy strategies to...
ORGANISM(S): Peptoclostridium difficile (strain 630) (Clostridium difficile) 
2018-10-25 | PXD010279 | Pride
Proteomics Relative protein quantification Mass spectrometry Metabolic labeling Clostridioides difficile 
GenomicsGel-free library preparation for next-generation RNA sequencing and small RNA quantification
Next-generation RNA sequencing (RNA-seq) is hampered by “primer dimer” (PD) artifacts and its quantitative performance reduced by polymerase fall-off (PF) at RNA modifications and secondary structures. Here we improved RNA-seq efficiency by incorporating (i) a post-reverse-transcription (RT) digesti...
ORGANISM(S): Escherichia coli 
2025-12-15 | GSE313501 | GEO
ProteomicsMedian Based Absolute Quantification using Fully Unlabelled Generic Internal Standard
By reporting molar abundances of proteins, absolute quantification determines their stoichiometry in complexes, pathways or networks and also relates them to abundances of non-protein biomolecules. Typically, absolute quantification relies either on protein- specific isotopically labelled peptide st...
ORGANISM(S): Homo sapiens (Human) Caenorhabditis elegans 
2021-11-30 | PXD026886 | Pride
Absolute quantification 
GenomicsGel-free library preparation for next-generation RNA sequencing and small RNA quantification
Gel-free library preparation for next-generation RNA sequencing and small RNA quantification
PRJNA1379658 | ENA
TranscriptomicsA proteomic view on the cell biology of Staphylococcus aureus proteome
The genome sequence is the “blue-print of life”, but proteomics is required to relate this blue-print of life to the actual physiology of living cells. Because of their low complexity – only about 2.000 proteins make a Staphylococcus aureus cell viable – bacteria are excellent model systems to ident...
ORGANISM(S): Staphylococcus aureus 
2009-11-11 | E-GEOD-15060 | biostudies-arrayexpress
ProteomicsHost Cell Protein Quantification Workflow Using Optimized Standards combined with Data-Independent Acquisition Mass Spectrometry
Monitoring of host cell proteins (HCPs) during the manufacture of monoclonal antibodies (mAb) has become a critical requirement to provide effective and safe drug product. ELISA assays are still the current gold standard for the quantification of protein impurities. However, this technique has sever...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2023-07-20 | PXD029305 | Pride
Hcp profiler Protein quantification Data validation. Data-independent acquisition (dia) Host cell proteins (hcps) 
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