Sort   by:  
 Page size 
ProteomicsChromosomal domain formation by archaeal SMC, a roadblock protein, and DNA structure
In this work, we performed DNA affinity purification of proteins that bind to biotinylated DNA probe, denoted as difL and difR. The enriched proteins were subjected to SDS-PAGE, in-gel tryptic digestion, followed by LC-MSMS.
ORGANISM(S): Thermococcus Kodakarensis 
2024-12-16 | PXD053073 | JPOST Repository
ProteomicsIdentification of proteins in SDS-PAGE gel by LC-MSMS
Recombinant antibodies were purified from culture supernatants derived from the Dictyostelium discoideum model and loaded into an SDS-PAGE electrophoresis gel. The electrophoretic profile of the anti-alpha tubulin, anti-CISD1, and anti-CD8β antibodies reveals a band at 35 kDa, likely corresponding t...
ORGANISM(S): Dictyostelium discoideum (Slime mold) 
2025-04-11 | PXD053343 | Pride
Dictyostelium discoideum Recombinant antibodies 
Proteomicsrp_int_selected
exp 3: MSMS RP intensity selected of a Multidimensional LC and gel MS/MS study
ORGANISM(S): Saccharomyces Cerevisiae 
2005-12-31 | PAe000160 | PeptideAtlas
ProteomicsGel-based LC-MSMS, the interactome of XAF1 in 293T cells
We utilized mass spectrometry to analyze the interacting proteins of XAF1 in 293T cells.
ORGANISM(S): Homo Sapiens 
2023-03-22 | PXD041021 |
ProteomicsGel-based LC-MSMS, the interactome of RNF114 in 293T cells
We utilized mass spectrometry to analyze the interactome of RNF114 in 293T cells.
ORGANISM(S): Homo Sapiens 
2023-08-07 | PXD044440 |
ProteomicsNUMB isoforms interactome study
HEK293 cells were transfected with NUMB-FLAG isoforms 1,2,3,4. anti-FLAG immunoprecipitationIP of all isoforms plus control sample were loaded on SDS-PAGE gel and CTRL plus ISOform1,2,3,4 lanes upon IP were processed by LC-MSMS analysis.
ORGANISM(S): Homo sapiens (Human) 
2026-06-15 | PXD064868 | Pride
Numb protein mass spectrometry 
ProteomicsHuman RKO cells LC-MSMS (gel-based proteomics)
Tumor cells evade T cell-mediated immunosurveillance via the interaction between programmed death-1 (PD-1) ligand 1 (PD-L1) on tumor cells and PD-1 on T cells. Strategies disrupting PD-1/PD-L1 have shown clinical benefits in various cancers. However, the limited response rate prompts us to investiga...
ORGANISM(S): Homo sapiens (Human) 
2021-08-15 | PXD027826 | Pride
Huamn Rko cells Lc-msms 
ProteomicsRat Kir4.1 interactome with the particular purpose to identify 10 kDa copurified proteins using LC-MSMS
The project aims to determine the interactome of full-length Kir4.1 overexpressed in HEK293 stable cells. The overexpressed proteins were purified, and the purified samples, along with three bands from a 1D SDS-PAGE gel, were analyzed by LC-MSMS after trypsin digestion. The primary goal was to ident...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD060340 | Pride
Lc-msms Hek293 stable cells Full length rat kir4.1 Lc8 copurification 
ProteomicsMass spectrometry of in-gel digests reveals differences in amino acid sequences of high-molecular-weight glutenin subunits in spelt and emmer compared to common wheat
High-molecular-weight glutenin subunits (HMW-GS) play an important role for the baking quality of wheat. The ancient wheats emmer and spelt differ in their HMW-GS pattern compared to modern common wheat and this might be one reason for their comparatively poor baking quality. The aim of this study w...
ORGANISM(S): Triticum Dicoccoides Triticum Aestivum Triticum Spelta 
2020-01-15 | PXD016342 | panorama
ProteomicsPhos-tag SDS-PAGE followed by LC-MS/MS analysis of in vitro phosphorylated Nab2, Foxk1, and HURP
Purified GST-fused Nab2, Foxk1, and HURP were incubated with ATP in the presence or absence of His-tagged active ERK in vitro, separated by Phos-tag SDS-PAGE, and visualized by CBB staining. The most shifted regions of protein bands on the Phos-tag-containing gels were excised, digested with trypsin...
ORGANISM(S): Mus Musculus (mouse) 
2022-03-03 | PXD028837 | JPOST Repository
Sort   by:  
 Page size