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ProteomicsGFP-Trap-IP-MS
To analyze proteins copurified with GFP-ALLO-1, worm eggs expressing GFP-ALLO-1 or GFP in the atg-11 background were lysed in co-IP buffer, and GFP-ALLO-1 or GFP was immunoprecipitated with GFP-Trap-agarose beads. Proteins on the beads were directly digested with Trypsin overnight. Supernatants were...
ORGANISM(S): Caenorhabditis Elegans 
2018-01-31 | PXD007847 | JPOST Repository
ProteomicsGFP-Trap-IP-phospho
To analyze phosphorylation sites of ALLO-1, worm eggs expressing GFP-ALLO-1 in the atg-11 or ikke-1 background were lysed in co-IP buffer, and GFP-ALLO-1 was immunoprecipitated with GFP-Trap-agarose beads. Proteins on the beads were directly digested with Trypsin overnight. Supernatants were collect...
ORGANISM(S): Caenorhabditis Elegans 
2018-01-31 | PXD007848 | JPOST Repository
ProteomicsGFP-Trap analysis of PKP1-WT-GFP and PKP3-WT-GFP in keratinocytes
To identify putative interaction partners for Plakophilin (PKP) 1 and 3 that might be involved in the regulation of UV-induced signaling pathways, we used PKP1 knockout (KO) keratinocytes (KCs) expressing either PKP1-WT-EGFP or PKP3-WT-EGFP. Prior to GFP-trap immunoprecipitations, these cells were l...
ORGANISM(S): Mus musculus (Mouse) 
2026-09-22 | PXD065254 | Pride
Plakophilin 3 Keratinocytes Gfp-trap Uv-irradiation Plakophilin 1 
ProteomicsComplementary proteomics strategies identify the ataxin-1 interactome in Neuro-2a cells
To identify protein partners of ataxin-1 in neuronal cells under control or stress conditions, we use complementary proteomics strategies of proximity-dependent biotin identification (BioID) and affinity purification (via GFP-trap pulldown) in Neuro-2a cells expressing epitope-tagged forms of ataxin...
ORGANISM(S): Mus musculus (Mouse) 
2018-10-18 | PXD010352 | Pride
Gfp-trap Ataxin-1 Bioid Interactome Neuro-2a Lc – msms 
ProteomicsGFP-CYYR1 interactome vs. GFP in HEK293 cells
Cell lysates from HEK293 cells individually transfected with GFP or GFP-CYYR1 were purified on GFP-Trap affinity resin and analyzed by quantitative label-free mass spectrometry. Experiments were performed in 5 biological replicates for each condition.
ORGANISM(S): Homo sapiens (Human) 
2024-07-25 | PXD050895 | Pride
Gfp-trap Cyyr1 Hek293 Interactome 
UnknownA protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in Drosophila.
Not available
S-EPMC64981 | biostudies-literature
TranscriptomicsIdentification of RNA targets of EB1 using RIP-seq in Drosophila ovaries
Identification of RNAs that bind to EB1-GFP using RNA-immunoprecipitation followed by sequencing. Ovaries from flies expressing EB1-GFP (and GFP alone as a control) were used to immunoprecipitate crosslinked RNA-EB1GFP complexes using GFP Trap beads. The experiment was performed with three biologica...
ORGANISM(S): Drosophila melanogaster 
2021-07-24 | E-MTAB-9078 | biostudies-arrayexpress
UnknownGAL4 GFP enhancer trap lines for analysis of stomatal guard cell development and gene expression.
Not available
S-EPMC3071773 | biostudies-literature
UnknownDevelopment and evaluation of a Gal4-mediated LUC/GFP/GUS enhancer trap system in Arabidopsis.
Not available
S-EPMC1164422 | biostudies-literature
UnknownCombining GAL4 GFP enhancer trap with split luciferase to measure spatiotemporal promoter activity in Arabidopsis.
Not available
S-EPMC7217008 | biostudies-literature
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