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UnknownSPRTN patient variants cause global-genome DNA-protein crosslink repair defects.
Not available
S-EPMC9867749 | biostudies-literature
GenomicsDNA-Protein Crosslink sequencing (DPC-seq) in RPE-1 cells treated with formaldehyde
Covalent DNA-protein crosslinks (DPC) are toxic DNA lesions that require repair by global-genome and replication-coupled pathways. How cells respond when RNA polymerases stall at DPCs during transcription is unknown. DPC-seq is new method for the genome-wide mapping of covalent DNA-protein adducts.
ORGANISM(S): Homo sapiens 
2024-02-24 | E-MTAB-12912 | biostudies-arrayexpress
GenomicsDNA-Protein Crosslink sequencing (DPC-seq) in RPE-1 cells treated with formaldehyde
DNA-Protein Crosslink sequencing (DPC-seq) in RPE-1 cells treated with formaldehyde
PRJEB73285 | ENA
UnknownA quantitative PCR-based assay reveals that nucleotide excision repair plays a predominant role in the removal of DNA-protein crosslinks from plasmids transfected into mammalian cells.
Not available
S-EPMC5811311 | biostudies-literature
ProteomicsGenetic and proteomic screens reveal perturbed nucleotide metabolism and DNA-protein crosslink formation as central mediators of decitabine cytotoxicity
Decitabine (5-aza-dC) is a DNMT1-DPC inducing agent used to treat several hematological cancers. However, response rates vary, relapse is common, and predictive biomarkers are unknown. Here, we develop an adaptation of identification of proteins on nascent DNA (iPOND) by combining EdU with 5-aza-dC ...
ORGANISM(S): Homo sapiens (Human) 
2024-04-18 | PXD045071 | Pride
Decitabine Dna-protein crosslink Topors Global-genome dpc repair Dna methylation Sumo Dnmt1 
ProteomicsIdentification of formaldehyde-induced DNA-protein crosslinks by PxP-MS
Compromised enzymatic actions, reactive metabolites, and various chemotherapeutics cause toxic covalent DNA-protein crosslinks (DPCs). Failure to repair DPCs results in genomic instability, premature aging, and tumorigenesis. However, understanding the principles underlying DPC formation and repair ...
ORGANISM(S): Homo sapiens (Human) 
2023-01-20 | PXD026654 | Pride
Formaldehyde Pxp Dna-protein crosslinks Sprtn 
UnknownAtypical genotoxicity of carcinogenic nickel(II): Linkage to dNTP biosynthesis, DNA-incorporated rNMPs, and impaired repair of TOP1-DNA crosslinks.
Not available
S-EPMC10692736 | biostudies-literature
TranscriptomicsPRMT5 protects genomic integrity during global DNA demethylation in primordial germ cells and preimplantation embryos
Primordial germ cells (PGCs) and preimplantation embryos undergo epigenetic reprogramming, which entails comprehensive erasure of DNA methylation. We found that PRMT5, an arginine methyltransferase, translocates from the cytoplasm to the nucleus during this process. Here we show that conditional los...
ORGANISM(S): Mus musculus 
2014-09-09 | E-GEOD-61216 | biostudies-arrayexpress
TranscriptomicsGene silencing triggers Polycomb Repressive Complex 2 recruitment to CpG islands genome-wide [RNA-seq_differentiation]
Polycomb group (PcG) proteins are required for normal differentiation and development, and their activity is found deregulated in cancer. PcG proteins are involved in gene silencing, however, whether they initiate or maintain transcriptional repression is a subject of debate. Here, we show that knoc...
ORGANISM(S): Mus musculus 
2014-07-03 | E-GEOD-58017 | biostudies-arrayexpress
TranscriptomicsGene silencing triggers Polycomb Repressive Complex 2 recruitment to CpG islands genome-wide [iTC-seq_drugs]
Polycomb group (PcG) proteins are required for normal differentiation and development, and their activity is found deregulated in cancer. PcG proteins are involved in gene silencing, however, whether they initiate or maintain transcriptional repression is a subject of debate. Here, we show that knoc...
ORGANISM(S): Mus musculus 
2014-07-03 | E-GEOD-58018 | biostudies-arrayexpress
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