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We present the adaptability of Mascot search engine for automated identification of intact glycopeptide mass spectra. The steps involved in adopting Mascot for intact glycopeptide analysis include: i) assigning unique one letter codes for monosaccharides, ii) linearizing glycan sequences and iii) pr...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-07-02 | MSV000082567 | MassIVE
In this study, we conducted a systematic investigation into a glycoproteomic workflow. Firstly, Label-free and TMT labeling strategies were utilized for intact N-glycopeptide quantification. A total of 2,924 unique intact N-glycopeptides can be quantified after being labeled using TMT approach. Comp...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2025-05-07 | PXD054874 | Pride
Multiple sclerosis biomarker discovery in pooled cerebrospinal fluid (CSF) using glycopeptide enrichment, TMT labeling and LC-MS/MS. Comparing 3 pools of CSF from relapsing-remitting MS (RRMS) patients to 3 pools of CSF from patients with other neurological diseases (OND). To reveal protein groups a...
ORGANISM(S): Homo sapiens (Human) 
2016-10-13 | PXD004540 | Pride
We report a LC-MS/MS O-glycoproteomics strategy using Data Independent Acquisition (DIA) mode that holds the potential for enabling direct analysis of O-glycoproteins with characterization of sites and structures of O-glycans on a proteome-wide scale with quantification of stoichiometries. To explor...
ORGANISM(S): Homo sapiens (Human) 
2019-11-19 | PXD011063 | Pride
In this project, the proximal proteins of the balhimycin transporter Tba in A. balhimycina were investigated in order to elucidate potential interaction partners. Tba is an ABC transporter, which is co-encoded with biosynthetic enzymes in the biosynthetic gene cluster of the glycopeptide antibiotic ...
ORGANISM(S): Bacteria Amycolatopsis balhimycina 
2025-05-07 | PXD054387 | Pride
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