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Analysis of U2AF2-TurboID-HA interactions by immunoprecipitation for comparison with proximity labeling. Cells expressing U2AF2 (iU2) fused with TurboID in either the undifferentiated state (D0) or after mesoderm differentiation (D1) were analyzed to identify the interactome and detect changes durin...
ORGANISM(S): Homo sapiens (Human) 
2025-04-13 | PXD062890 | Pride
Development a novel system employing TurboID-fusion proteins within an integrative and inducible expression vector to investigate the interactome during stem cell differentiation. Cells expressing U2AF2 (iU2) or GFP (iGFP) fused with TurboID in undifferentiated (D0) or mesoderm differentiated (D1) w...
ORGANISM(S): Homo sapiens (Human) 
2025-01-07 | PXD056299 | Pride
The label-free quantitative mass spectrometry methods, in particular the SWATH-MS approach, have gained popularity and became a powerful technique for comparison of large datasets. In the present work, it is introduced the use of recombinant proteins as internal standards for untargeted label-free m...
ORGANISM(S): Aequorea victoria Homo sapiens (Human) Escherichia coli Rattus norvegicus (Rat) 
2019-08-19 | PXD009068 | Pride
gd T cells are major innate sources of interleukin-17 (IL-17) and interferon-g (IFN-g), which are differentially produced by two thymically-derived subsets segregated on CD27 expression. However, the molecular mechanisms that program the functional differentiation of gd cells remain incompletely und...
ORGANISM(S): Mus musculus 
MicroRNAs (miRNAs) are a new class of small RNAs of approximately 22 nucleotides in length that control eukaryotic gene expression by fine tuning mRNA translation. They regulate a wide variety of biological processes, namely developmental timing, cell differentiation, cell proliferation, immune resp...
ORGANISM(S): Danio rerio 
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