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The mRNA 5′ cap is normally essential for eukaryotic mRNA translation, stabilization and transport and both the cap and eIF4E are important elements of post-transcriptional gene regulation. To further our understanding of mRNA translation in the human malaria parasite Plasmodium falciparum, we hav...
ORGANISM(S): Plasmodium falciparum 
A series of trinucleotide cap analogs functionalized with an amine-terminated linker at the guanosine ribose and immobilized on BrCN-activated Sepharose was prepared. These affinity resins AR-1 (Am), AR-2 (m6Am), and AR-3 (Bn6Am) were incubated with HEK293F cell extract in the presence of GTP to lim...
ORGANISM(S): Homo sapiens (Human) 
2024-03-08 | PXD046838 | Pride
An experiment was performed to compare global transcription patterns in two tissues of Lepidium sativum seeds at different times during imbibition leading up to, but not including, radicle protrusion from the seed (e.g. germination). RNA extractions from the two tissues were hybridised to CATMA Arab...
ORGANISM(S): Lepidium sativum 
This dataset was acquired to identify human proteins binding to tetraphosphate mRNA cap analogs. Affinity resins were derivatized with tetraphosphate mRNA cap analogs or a trinucleotide cap control and affinity purification performed in HEK293F cell extracts. The pulled-down proteins were eluted us...
ORGANISM(S): Homo sapiens (Human) 
2024-10-02 | PXD053198 | Pride
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