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This dataset was acquired to identify human proteins binding to tetraphosphate mRNA cap analogs. Affinity resins were derivatized with tetraphosphate mRNA cap analogs or a trinucleotide cap control and affinity purification performed in HEK293F cell extracts. The pulled-down proteins were eluted us...
ORGANISM(S): Homo sapiens (Human) 
2024-10-02 | PXD053198 | Pride
A series of trinucleotide cap analogs functionalized with an amine-terminated linker at the guanosine ribose and immobilized on BrCN-activated Sepharose was prepared. These affinity resins AR-1 (Am), AR-2 (m6Am), and AR-3 (Bn6Am) were incubated with HEK293F cell extract in the presence of GTP to lim...
ORGANISM(S): Homo sapiens (Human) 
2024-03-08 | PXD046838 | Pride
Human Pgp with a C-terminal MBP tag was expressed in HEK293F cells, and the pulldown samples were analyzed by mass spectrometry.
ORGANISM(S): Homo Sapiens 
The plasmids were then transiently transfected into HEK293F expression system for overexpression. After incubation for 48 h, the cells were collected, washed one with ice-cold PBS. The resuspended cells were then lysed by sonication, and the lysate supernatant after centrifugation was incubated with...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-05-15 | MSV000091953 | MassIVE
BRCA1-associated protein 1 (BAP1) is a multidomain deubiquitinase (DUB) with a ubiquitin carboxyl-terminal hydrolase (UCH) domain at its N-terminus. BAP1 mainly localizes in the nucleus and primarily functions as a transcriptional regulator in diverse cellular pathways, including cell proliferation...
ORGANISM(S): Homo sapiens (Human) 
2022-04-14 | PXD030044 | Pride
Genomics
rAAV5 screening in HEK293F cells
Genomics
rAAV4 screening in HEK293F cells
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