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ProteomicsMitochondrial fatty acid synthesis coordinates oxidative metabolism in mammalian mitochondria
Cells harbor two systems for fatty acid synthesis, one in the cytoplasm (catalyzed by fatty acid synthase, FASN) and one in the mitochondria (mtFAS). In contrast to FASN, mtFAS is poorly characterized, especially in higher eukaryotes, with the major product(s), metabolic roles, and cellular function...
ORGANISM(S): Homo sapiens (Human) 
2020-09-02 | PXD021034 | Pride
Metabolism Mitochondria Electron transport chain Fatty acid synthesis Oxphos Respiration Reductive carboxylation Lipoylation tca cycle Muscle 
ProteomicsProteome-wide quantitative multiplexed profiling of protein expression: Carbon source dependency in S. cerevisiae
The global proteomic alterations in the budding yeast Saccharomyces cerevisiae due to differences in carbon sources can be comprehensively examined using mass spectrometry-based multiplexing strategies. Here we investigate changes in the S. cerevisiae proteome resulting from cultures grown in minima...
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) 
2020-01-30 | MSV000084868 | MassIVE
ProteomicsThe role of Cdk1-cyclin B in G1/S transition revealed by chemical genetic inhibition of Cdc7
Entry of mammalian cells into DNA synthesis (S-phase) represents a key event in cell division. According to the current cell cycle models, the Cdc7 kinase represents the essential and rate-limiting trigger of DNA replication, acting together with the cyclin-dependent kinase Cdk2. Using chemical gene...
ORGANISM(S): Mus musculus (Mouse) 
2022-03-11 | PXD025625 | Pride
Cdk1-cyclinb G1/s transition Cdc7 Dna replication 
ProteomicsVRK1 is required in VRK2-methylated cancers of the nervous system
Collateral lethality occurs when loss of one paralog renders cancer cells dependent on the remaining paralog. Combining genome scale CRISPR/Cas9 screens coupled with RNA-sequencing in over 900 cancer cell lines, we found that cancers of nervous system lineage, including adult and pediatric gliomas a...
ORGANISM(S): Homo sapiens (Human) 
2022-09-06 | PXD030599 | Pride
Phosphorylation 
TranscriptomicsTra1 has specific regulatory roles, rather than global functions, within the SAGA coactivator complex
The SAGA complex is a conserved, multifunctional coactivator that plays broad roles in eukaryotic transcription. Previous studies suggested that Tra1, the largest SAGA component, is required either for SAGA assembly or for recruitment by DNA-bound transcriptional activators. In contrast to S. cere...
ORGANISM(S): Schizosaccharomyces pombe 
2011-06-13 | E-MTAB-678 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of fission yeast SAGA mutants shows that the SAGA complex controls the switch from proliferation to sexual differentiation through the opposing roles of its subunits Gcn5 and Spt8
The SAGA complex is a conserved multifunctional coactivator known to play broad roles in eukaryotic transcription. To gain new insights into its functions, we have performed biochemical and genetic analyses of SAGA in the fission yeast, Schizosaccharomyces pombe. Purification of the S. pombe SAGA ...
ORGANISM(S): Schizosaccharomyces pombe 
2008-11-19 | E-MTAB-53 | biostudies-arrayexpress
ProteomicsTMT-based Multiplexed (Chemo)proteomics on the Orbitrap Astral Mass Spectrometer
This dataset contains an evaluation of TMT-based multiplexed proteomics on the Orbitrap Astral mass spectrometer.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-12-17 | MSV000096674 | MassIVE
ProteomicsIntegrated Proteogenomic Characterization across Major Histological Types of Pediatric Brain Cancer

Francesca Petralia,Nicole Tignor,Boris Reva, et al., (2020) Cell 183, 1962-1985

We report a comprehensive proteogenomics analysis, including whole-genome sequencing, RNA sequencing, and proteomics and pho...

ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-02-04 | MSV000086810 | MassIVE
ProteomicsNon-coding divergence of the human X and Y chromosomes drove male-biased expression of dosage-sensitive regulators
Little is known about how Y-chromosome gene expression directly contributes to differences between XX (female) and XY (male) individuals in non-reproductive tissues. It is often said that Y-chromosome genes show low expression outside the testis. Here, we analyzed quantitative profiles of Y-chromoso...
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD017055 | Pride
Tandem mass tags Proteomics Orbitrap lumos Lc-ms/ms 
ProteomicsProteome-wide evaluation of two common protein quantification methods
Proteomics experiments commonly aim to estimate and detect differential abundance across all expressed proteins. Within this experimental design, some of the most challenging measurements are small fold changes for lower abundance proteins. While bottom-up proteomics methods are approaching comprehe...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Saccharomyces Cerevisiae (ncbitaxon:4932) 
2019-02-15 | MSV000083453 | MassIVE
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