DJ-1 expression protects H157 cells from cell death, using siRNA oligomers we knocked down DJ-1 and evaulated gene expression using Affymetrix U133A GeneChip arrays. Experiment Overall Design: H157 cells were transfected every 24 hours with siRNA oligomers for 3 consecutive days, lysed 96 hours afte...
H157 cells were treated with nanocomposite AANL-SeNPs and harvested for mass spectrometry detection. The mass spectrum data were obtained by timsTOF Pro combined with UltiMate 3000 RSLCnano liquid chromatograph system. DIA-NN software is used to analyze DIA original data files. The differentially ex...
To development our gene expression approach, we have employed whole genome microarray expression profiling as a discovery platform to identify genes potentialy regulated by the polarity protein PAR3. Human SCC cell line, NCI-H157 (H157), has a big deletion at PARD3 locus and showed no PAR3 protein e...
H157 cells transfected with vector and overexpressed AACT plasmids were treated and used for MS detection. The MS data were acquired using an Orbitrap Exploris 480 Mass Spectrometer coupled with an EASY-NLC 1200 liquid phase LC-MS system. DIA raw data files were analyzed by DIA-NN software. The diff...
Methylating agents of SN1 type constitute a widely used class of anticancer drugs, the effect of which on human non-small-cell lung cancer (NSCLC) has not been adequately studied. We thus studied the effect of N-methyl-N-nitrosourea (MNU), a model SN1 methylating agent, on two human NSCLC cell lines...
The dismal lethality of lung cancer is due to late stage at diagnosis and inherent therapeutic resistance. The incorporation of targeted therapies has modestly improved clinical outcomes, but the identification of new targets could further improve clinical outcomes by guiding stratification of poor-...
DJ-1 expression protects H157 cells from cell death, using siRNA oligomers we knocked down DJ-1 and evaulated gene expression using Affymetrix U133A GeneChip arrays. Keywords: siRNA comparison
To development our gene expression approach, we have employed whole genome microarray expression profiling as a discovery platform to identify genes potentialy regulated by the polarity protein PAR3. Human SCC cell line, NCI-H157 (H157), has a big deletion at PARD3 locus and showed no PAR3 protein e...
