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TranscriptomicsSmall RNAs in Chlamydomonas reinhardtii
RNA populations in Chlamydomonas reinhardtii Keywords: Highly parallel pyrosequencing Small RNAs were prepared from Chlamydomonas reinhardtii total extracts,ligated to a 3' adaptor and a 5' acceptor sequentially, and then RT-PCR amplified. PCR products were reamplified using a pair of 454 cloning pr...
ORGANISM(S): Chlamydomonas reinhardtii 
2007-05-15 | E-GEOD-7308 | biostudies-arrayexpress
TranscriptomicsProbing the initiation and effector phases of the somatic piRNA pathway in Drosophila
Combining RNAi in cultured cells and analysis of mutant animals, we probed roles of known piRNA pathway components in the initiation and effector phases of transposon silencing. total RNA and RNA associated with Piwi was isolated and size-fractionated by PAGE into 19-29nt. These were processed and s...
ORGANISM(S): Drosophila melanogaster 
2010-10-25 | E-GEOD-24108 | biostudies-arrayexpress
TranscriptomicsCharacterization of active piRNAs and an piRNA machinery in WRR-1 cells deficient for the Hippo pathway (deficient for the warts tumor suppressor) and expressing activated RasV12 (small RNA)
We have found that oncogenic Ras combined with loss of the Hippo tumour-suppressor pathway reactivates a primary piRNA pathway in Drosophila somatic cells, coincident with oncogenic transformation. In these cells, Piwi becomes loaded with piRNAs derived from generative loci, which are normally restr...
ORGANISM(S): Drosophila melanogaster 
2016-08-01 | E-GEOD-83871 | biostudies-arrayexpress
ProteomicsMaternally inherited piRNAs silence transposons during Drosophila embryogenesis
The piRNA pathway controls transposon expression in animal germ cells, thereby ensuring genome stability over generations. piRNAs are maternally deposited and required for proper transposon silencing in adult offspring. However, a long-standing question in the field is the precise function of matern...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2021-07-26 | PXD022409 | Pride
Drosophila Embryogenesis Pirna Tmt-ms 
ProteomicsSpecialization of the Drosophila nuclear export family protein, Nxf3, for piRNA precursor export
The piRNA pathway is a conserved small RNA-based immune system that protects animal germ cell genomes from the harmful effects of transposon mobilisation. In Drosophila ovaries, most piRNAs originate from dual-strand clusters, which generate piRNAs from both genomic strands. Dual-strand clusters use...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2019-07-29 | PXD014472 | Pride
Pirna rna export piwi drosophila transposons 
ProteomicspiRNA-guided co-transcriptional silencing coopts nuclear export factors
The PIWI-interacting RNA (piRNA) pathway is a small RNA-based immune system that controls the expression of transposons and maintains genome integrity in animal germlines1,2. In Drosophila, piRNA-guided silencing is achieved, in part, via co-transcriptional repression of transposons by Piwi. This de...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2019-06-20 | PXD011415 | Pride
Pirna transposons piwi nuclear export 
TranscriptomicsCharacterization of active piRNAs and piRNA machinery in WRR-1 cells deficient for the Hippo pathway (deficient for the warts tumor suppressor) and expressing activated RasV12 (RNA-seq)
We have found that oncogenic Ras combined with loss of the Hippo tumour-suppressor pathway reactivates a primary piRNA pathway in Drosophila somatic cells, coincident with oncogenic transformation. In these cells, Piwi becomes loaded with piRNAs derived from generative loci, which are normally restr...
ORGANISM(S): Drosophila melanogaster 
2016-08-01 | E-GEOD-83843 | biostudies-arrayexpress
ProteomicsRequirement of Nuclear Pore Complex subunits for transposon silencing in Drosophila
The piRNA pathway is a small RNA-based immune system that silences mobile genetic elements in animal germlines. In Drosophila ovaries, piRNAs are produced from discrete genomic loci, called piRNA clusters, which are composed of inactive transposon copies and fragments and thus constitute a genetical...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2021-04-16 | PXD019671 | Pride
Pirna piwi npc nuclear export 
ProteomicsRequirement of Nuclear Pore Complex Subunits for transposon silencing in Drosophila
The piRNA pathway is a small RNA-based immune system that silences mobile genetic elements in animal germlines. In Drosophila ovaries, piRNAs are produced from discrete genomic loci, called piRNA clusters, which are composed of inactive transposon copies and fragments and thus constitute a genetical...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2021-04-15 | PXD019670 | Pride
Pirna piwi npc nuclear export 
ProteomicsDaedalus and Gasz recruit Armitage to mitochondria, bringing piRNA precursors to the biogenesis machinery
The piRNA pathway is a small RNA-based immune system that silences mobile genetic elements in animal germlines. piRNA biogenesis requires a specialised machinery that converts long single-stranded precursors into small RNAs of ~25-nucleotides in length. This process involves factors that operate in ...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2019-04-18 | PXD013417 | Pride
Pirna piwi drosophila 
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