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ProteomicsNleB2 from Enteropathogenic Escherichia coli is an arginine-glucose transferase
Within this study we demostrate that NleB2 from Enteropathogenic Escherichia coli is a arginine-glucose transferase. Using in vitro and in vivo assays we demostrate that control of the utelisation of UDP-GlcNAc or UDP-Glc is controlled by a single amino acid.
ORGANISM(S): Homo sapiens (Human) 
2021-05-25 | PXD021796 | Pride
Ethcd Epec Arginine glycosylation Effectors 
ProteomicsConfirmation of Arg-Glucosylation on multiple Death domain proteins
In vitro and tranfection analysis of death domain containing proteins to assess the ability of NleB2 to glycosylate these proteins.
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2021-05-25 | PXD025531 | Pride
Lc-ms Arginine glycosylation Nleb2 
Proteomicsidentification of the endogenous insoluble host targets of the Salmonella effectors SSeK3
Strains of Salmonella utilise two distinct type three secretion systems to deliver effector proteins directly into host cells. The Salmonella effector SseK3 is a arginine glycosyltransferase that modify mammalian proteins with N-acetyl glucosamine (GlcNAc). Here, we used Arg-GlcNAc glycopeptide immu...
ORGANISM(S): Mus musculus (Mouse) 
2020-07-28 | PXD015082 | Pride
Glycosylation Salmonella Proteomics Arg-glcnac Ssek3 
Proteomicsidentification of the endogenous host targets of the Salmonella effectors SSeK1 and 3
Strains of Salmonella utilise two distinct type three secretion systems to deliver effector proteins directly into host cells. The Salmonella effectors SseK1 and SseK3 are arginine glycosyltransferases that modify mammalian death domain containing proteins with N-acetyl glucosamine (GlcNAc) when ove...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344 
2019-03-27 | PXD010769 | Pride
Glycosylation Salmonella Proteomics Infection Glycopeptide enrichment 
TranscriptomicsControl of Virulence Gene Expression in Citrobacter rodentium by Trascription Factor RegA
Identification of the targets of RegA with and without bicarbonate stimulation by comparing RegA knockout to multicopy RegA transgenics. RegA is an AraC like transcription factor identified in a mutational screen for virulence genes in Citrobacter rodentium, an attaching and effacing pathogen that ...
ORGANISM(S): Citrobacter rodentium 
2010-05-19 | E-GEOD-12876 | biostudies-arrayexpress
ProteomicsNleH LFQ phoshoproteome analysis (EThcD runs)
Using Phosphopeptide enrichment and MS analysis the effect of NleH1 and NleH2 on the phosphoproteome of host cells was investigated - EThcD runs of samples
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2022-08-07 | PXD031090 | Pride
Human Phosphorylation Epec 
ProteomicsNleH1/2 proteomic analysis scripts
Combined script for the generation of images within paper. Three experiments in total 1) Phosphopeptide enrichment of Three biological replicates of each condition (uninfected, WT infected , detla NleH1 NleH2 infected, pNleH1 infected and pNleH2 infection) 2) Total proteome analysis to complement ph...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2022-08-07 | PXD031117 | Pride
Epec Nleh2 Nleh1 Phosphorylation 
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