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TranscriptomicsTranscriptome-wide profiling of Spadetail-regulated genes in early somite progenitors
We integrate zebrafish embryology with photoactivatable caged morpholinos (cMO), the photoactivatable lineage tracer caged fluorescein-dextran (cFD), fluorescence-activated cell-sorting (FACS) and RNA sequencing (RNA-seq) to identify Spadetail-regulated genes in mesodermal cells enriched for early s...
ORGANISM(S): Danio rerio 
2016-06-27 | E-GEOD-70623 | biostudies-arrayexpress
UnknownCore promoter T-blocks correlate with gene expression levels in C. elegans.
Not available
S-EPMC3083087 | biostudies-literature
TranscriptomicsSingle-cell RNA sequencing data (SORT-seq) of Treg cells in the human postnatal thymus
We have performed single-cell RNA sequencing to investigate human regulatory T cell development in the thymus. Treg cells were isolated from a postnatal thymus derived from a 7-week old infant. We examined the composition of the Treg cell compartment in the human thymus and aimed to dissect developm...
ORGANISM(S): Homo sapiens 
2021-11-08 | E-MTAB-11019 | biostudies-arrayexpress
TranscriptomicsSpatiotemporal embryonic transcriptomics reveals the evolutionary history of the endoderm germ layer
The germ layer concept has been one of the foremost organizing principles in developmental biology, classification, systematics and evolution for 150 years. Of the three germ layers, the mesoderm is found in bilaterian animals but is absent in species in the phyla Cnidaria and Ctenophora, which has ...
ORGANISM(S): Caenorhabditis elegans 
2014-12-11 | E-GEOD-50548 | biostudies-arrayexpress
TranscriptomicsTechnical Variations in Low-Input RNA-seq Methodologies
Transcriptomics data obtained from limiting amounts of mRNA is often noisy, providing primarily qualitative changes in transcript expressions. So far, technical variations arising out of the library preparation protocols have not been adequately characterized at reduced levels of mRNA. Here, we gene...
ORGANISM(S): Mus musculus 
2014-01-13 | E-GEOD-50856 | biostudies-arrayexpress
TranscriptomicsLymphatic vessels arise from a niche of multipotent angioblasts within the floor of the cardinal vein
How cells acquire their fate is a fundamental question in both developmental and regenerative biology. Multipotent progenitors undergo gradual cell fate restriction in response to temporal and positional cues from the microenvironment, the nature of which is far from being clear. In the case of the ...
ORGANISM(S): Danio rerio 
2015-05-21 | E-GEOD-65751 | biostudies-arrayexpress
ProteomicsProteome Characterization of HFFs Upon NORAD lncRNA Knockdown
Mass spectrometry data were processed using MSFragger via FragPipe with default parameters. For downstream analysis, Perseus was used. The MaxLFQ intensity values were log2-transformed and filtered to include only proteins detected in at least two replicates. Missing values were imputed from a norma...
ORGANISM(S): Homo sapiens (Human) 
2024-12-23 | PXD056160 | Pride
Norad Interferon Hff Lncrna Stat3 
TranscriptomicsAmphimedon queenslandica high resolution developmental transcriptomic time-course
Classical embryological studies revealed that during mid-embryogenesis vertebrates show similar morphologies. This “phylotypic stage” has recently received support from transcriptome analyses, which have also detected similar stages in nematodes and arthropods. A conserved stage in these three p...
ORGANISM(S): Amphimedon queenslandica 
2016-02-18 | E-GEOD-60483 | biostudies-arrayexpress
TranscriptomicsMnemiopsis leidyi high resolution developmental transcriptomic time-course
Classical embryological studies revealed that during mid-embryogenesis vertebrates show similar morphologies. This “phylotypic stage” has recently received support from transcriptome analyses, which have also detected similar stages in nematodes and arthropods. A conserved stage in these three p...
ORGANISM(S): Mnemiopsis leidyi 
2016-02-18 | E-GEOD-60478 | biostudies-arrayexpress
GenomicsDeciphering the regulatory landscape of fetal and adult gd T-cell development at single-cell resolution
Deciphering the regulatory landscape of fetal and adult gd T-cell development at single-cell resolution
PRJNA475973 | ENA
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