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MetabolomicsNorbergenin prevents LPS-induced inflammatory responses in macrophages through inhibiting NFkB, MAPK and STAT3 activation and blocking metabolic reprogramming

Inflammation is thought to be a key cause of many chronic diseases and cancer. However, current therapeutic agents to control inflammation have limited long-term use potential due to various side-effects. This study aimed to examine norbergenin, a constituent of traditional anti-inflammatory reci...

2023-04-06 | MTBLS7127 | MetaboLights
TranscriptomicsIdentification of IL-21-induced STAT3 dependent genes in human B cells
IL-21 induces B cell activation, and differentiation into antibody-secreting plasmablasts in vitro. This process is abolished by loss-of function mutations in STAT3 We used microarrays to identify genes that are induced by IL-21 in a STAT3-dependent manner Sort-purified naïve B cells from four heal...
ORGANISM(S): Homo sapiens 
2014-01-09 | E-GEOD-51587 | biostudies-arrayexpress
GenomicsThe Stat3/GR interaction code: predictive value of direct/indirect DNA recruitment for transcription outcome
Transcription factor recruitment to genomic sites of action is primarily due to direct protein:DNA interactions. The subsequent recruitment of co-regulatory complexes leads to either transcriptional activation or repression. In contrast to this canonical scheme, some transcription factors such as th...
ORGANISM(S): Mus musculus 
2012-06-06 | E-GEOD-37235 | biostudies-arrayexpress
TranscriptomicsGene expression profiles regulated by Stat5a/b vs. Stat3 in human prostate cancer cells.
Identification of the molecular changes that promote viability and metastatic behaviour of prostate cancer cells is critical for the development of improved therapeutic interventions for prostate cancer. Stat5a/b and Stat3 are both constitutively active in locally-confined and advanced prostate canc...
ORGANISM(S): Homo sapiens 
2010-05-27 | E-GEOD-17482 | biostudies-arrayexpress
ProteomicsDUSP2 dephosphorylates STAT3 at Y705 and S727 residues
In the related study, to determine whether DUSP2 definitively served as a phosphatase for STAT3, an in vitro phosphatase assay was used. Using S-tag beads, p-STAT3 was pulled down from IL-6 stimulated HEK293T cells transfected with STAT3-S-Tag. DUSP2 or control protein, which was purified from HEK29...
ORGANISM(S): Homo sapiens (Human) 
2015-10-13 | PXD002743 | Pride
Dusp2 Phosphorylation Stat3 
TranscriptomicsGenome wide STAT3 RNAi expression profiling data from Thp,Th0 and Th17 cells
STAT3 is an immidiate regulator of Th17 differentiation. STAT3 difieciency downmodulate Th17 specific genes and Th17 responses. Therefore, we intend to identify genome wide targets of STAT3. We used microarrays to profile gene expression of STAT3 regulated genes during Th17 polarization. Total RNA w...
ORGANISM(S): Homo sapiens 
2017-05-03 | E-GEOD-67185 | biostudies-arrayexpress
TranscriptomicsExpression data from regulatory T cells with Stat3 ablation
We used microarrays to detail the global programme of gene expression dependent upon Stat3 in regulatory T cells Regulatory T cells isolated from Foxp3 Stat3 or Foxp3 Stat3 were isolated using FACS based sorting followed by RNA extraction, amplification, labeling and...
ORGANISM(S): Mus musculus 
2009-09-04 | E-GEOD-17962 | biostudies-arrayexpress
TranscriptomicsIL6-STAT3 downstream targets in MCF10A/MCF10A.ErbB2* cells
Overexpression in IL6 in MCF10A vs. WT, MCF10A.ErbB2* with/without shRNA targeting STAT3 Overexpression in IL6 in MCF10A vs. WT, MCF10A.ErbB* with/without shSTAT3, duplicates, two STAT3 shRNAs
ORGANISM(S): Homo sapiens 
2015-09-03 | E-GEOD-62235 | biostudies-arrayexpress
ProteomicsMass spectrometry analysis of proteins interacting with STAT3
Mass spectrometry analysis of proteins interacting with STAT3¬Flag was performed. STAT3-Flag was transiently expressed in HeLa cells and the interactome of STAT3-Flag was studied in lysosomal extracts. proteins co-¬immunoprecipitating with STAT3--Flag identified eight subunits of the vacuolar H+-¬ A...
ORGANISM(S): Homo sapiens (Human) 
2018-08-29 | PXD006788 | Pride
Co-immunoprecipitation Stat3 
GenomicsGenome-wide binding of STAT3 in peritoneal macrophages
To try to identify the mechanism of STAT3’s indirect action we have used a genomic approach to map the binding sites of STAT3 within the genome and also used RNA-seq technology to map the changes in RNA expression and transcript isoforms in response to IL-10. Examination of STAT3 binding by ChIP-s...
ORGANISM(S): Mus musculus 
2012-02-13 | E-GEOD-31529 | biostudies-arrayexpress
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