Sort   by:  
 Page size 
ProteomicsCellular ADP-ribosylome characterization with HCD and EThcD
Protein ADP-ribosylation is a post-translational modification involved in important physiological and pathological processes. Here, we present an optimized method using the Orbitrap Fusion Tribrid mass spectrometer, which drastically improves the overall ADP-ribosylation site localization score and ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-07-28 | MSV000080008 | MassIVE
ProteomicsCharacterization of HLA-A*02:01 MHC Immunopeptide Antigens Enhanced by Ultraviolet Photodissociation Mass Spectrometry
Triplicate results for 193 nm UVPD, HCD, and EThcD of a mixture of 157 synthetic peptide mixture, and 193 nm UVPD, HCD, and EThcD of BB7.2 and W6/32 immunoprecipitations
ORGANISM(S): Homo Sapiens (human) 
2021-08-13 | PXD027830 | JPOST Repository
ProteomicsGlobal-scale enrichment and identification of O-GlcNAc modified peptides in Arabidopsis seedling (S1 Part 1: HCD and EThcD part from Eclipse)
O-GlcNAcylation is a crucial post-translational modification of proteins observed in both plants and animals and plays a key role in growth and development. However, our understanding of this PTM in plants lags behind animals. To bridge this knowledge gap, we performed a global scale enrichment of O...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-05-21 | PXD044701 | Pride
Mass spectrometry O-glcnacylation Proteomics. Lectin weak affinity chromatography (lwac) 
UnknownImproving data quality and preserving HCD-generated reporter ions with EThcD for isobaric tag-based quantitative proteomics and proteome-wide PTM studies.
Not available
S-EPMC5509462 | biostudies-literature
ProteomicsComparison of affinity enrichment methods for O-GlcNAc proteomics
In this work, by using the lysates of PANC-1 (a pancreatic cancer cell line), we provided a head-to-head comparison of three affinity enrichment methods/materials (i.e., antibody, lectin AANL6, and an OGA mutant) for O-GlcNAc proteomics. The enrichment was performed before or after high-pH HPLC frac...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Saccharomyces Cerevisiae (ncbitaxon:4932) 
2024-04-23 | MSV000094604 | MassIVE
ProteomicsMHC immunopeptidome of JY cells analysed by EThcD or HCD at Orbitrap Fusion after mild acid elution
To understand and treat immunology-related diseases, a comprehensive, unbiased characterization of major histocompatibility complex (MHC) peptide ligands is of key importance. Preceding the analysis by mass spectrometry, MHC peptide ligands are typically isolated by MHC immunoaffinity chromatography...
ORGANISM(S): Homo sapiens (Human) 
2020-11-11 | PXD012437 | Pride
Post-translational modification Ethcd Hcd Hla ligandome Mild acid elution Cysteinylation Mhc bound peptides 
ProteomicsEnhanced Characterization of Lysine-Linked Antibody Drug Conjugates Enabled by Middle-Down Mass Spectrometry
HCD Triggered UVPD and EThcD files for characterization of trastuzumab-emtansine (T-DM1)
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-08-03 | MSV000092585 | MassIVE
ProteomicsDe Novo Sequencing of SARS-CoV-2 and influenza monoclonal antibodies by mass spectrometry using HCD and EThcD fragmentation and Supernovo software
In this study, we used electron-transfer/higher energy collision dissociation (EThcD) fragmentation to develop a mass-spectrometric (MS) method for distinguishing between Ile and Leu in Bovine Serum Albumin (BSA) and a SARS-CoV-1 anti-receptor binding domain (RBD) Spike monoclonal antibody (mAb). Su...
ORGANISM(S): Homo sapiens (Human) 
2022-06-03 | PXD030094 | Pride
Ethcd Monoclonal antibodies De novo sequencing 
ProteomicsMS-based sequencing of the anti-FLAG-M2 antibody
We demonstrate a method for direct de novo sequencing of monoclonal IgG from the purified antibody products. The method uses a panel of multiple complementary proteases to generate suitable peptides for de novo sequencing by LC-MS/MS in a bottom-up fashion. Furthermore, we apply a dual fragmentation...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2021-01-11 | PXD023419 | Pride
Antibody Stepped hcd Ethcd De novo sequencing 
ProteomicsAn evaluation of high-field asymmetric-waveform ion mobility spectrometry coupled to electron-transfer/higher-energy collision dissociation for ADP-ribosylation proteomics
Objective: ADP-ribosylation is a post-translational modification that plays an important role in cellular processes. Our previous work implemented multiple gas-phase separation strategies (e.g., FAIMS) and in-source CID on the quadrupole-Orbitrap (Exploris 480) to increase the yield and acceptor sit...
ORGANISM(S): Homo sapiens (Human) 
2026-04-13 | PXD067389 | Pride
Macrophage Proteomics Ethcd Hcd Adp-ribosylation 
Sort   by:  
 Page size