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TranscriptomicsHost cell responses to persistent mycoplasmas--different stages in infection of HeLa cells with Mycoplasma hominis
M. hominis adherence, colonisation and invasion of HeLa cells were characterised in a time-course study using microarray-based analysis of the HeLa cell transcriptome: 0 h to monitor the baseline of transcription, 4 h to examine host reactions to mycoplasma attachment, 48 h to capture in addition th...
ORGANISM(S): Homo sapiens 
2014-02-06 | E-GEOD-54698 | biostudies-arrayexpress
ProteomicsConfetti: A Multi-protease Map of the HeLa Proteome for Comprehensive Proteomics
Tryptic digestion proteomics gives limited coverage of many protein sequences as many proteins produce tryptic peptides that cannot be observed easily. Confetti is our comprehensive map of coverage for the HeLa proteome, using 48 different digests and CID/HCD LC-MS/MS analyses. An associated dataset...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-10-12 | MSV000081607 | MassIVE
ProteomicsPhosphopeptide enrichment on synthetic libraries and HeLa cells digest
2 phosphopeptide enrichment methods (TiO2 and Ti-IMAC) were evaluated on synthetic libraries and HeLa cells digest. The resulting peptides were compared to the ones without enrichment with regards to the nature of the phosphorylation site (serine, threonine, tyrosine) and the characteristics of the ...
ORGANISM(S): Homo sapiens (Human) 
2014-08-11 | PXD000759 | Pride
Phosphopeptide enrichment Tio2 Ti-imac Hela Synthetic library 
ProteomicsmTRAQ labeled natural proteome from HeLa
To identify proteotypic peptides, HeLa cells digest labeled with mTRAQdelta0 were extensively fractionated and analyzed by LC-MS/MS.
ORGANISM(S): Homo sapiens (Human) 
2016-12-07 | PXD001396 | Pride
Hela proteome Lc-ms/ms 
ProteomicsEvaluation of different data-driven rescoring platforms to increase peptide identifications in HeLa digest samples
Evaluate the performance of different rescoring platforms with the aim of standardizing pipelines within our research group.
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD052875 | Pride
Hela samples Rescoring pipelines 
ProteomicsProtein abundance estimation in HeLa cells
To estimate the amount of proteins in HeLa, whole cell digests were subjected to fractionation by off line reverse phase chromatography at basic pH followed by LC-MS/MS analysis
ORGANISM(S): Homo sapiens (Human) 
2016-12-07 | PXD001441 | Pride
Hela proteome Lc-ms/ms 
ProteomicsHeLa benchmark datasets DirectMS1 MSGF+
This technical dataset contains LC-MS runs of HeLa protein digest analyzed with a common shotgun proteomics pipeline based on MSGF+ and Percolator and DirectMS1 pipeline.
ORGANISM(S): Homo sapiens (Human) 
2023-09-25 | PXD045495 | Pride
Hela Proteomics Technical Directms1 
OtherConfetti: A Multi-protease Map of the HeLa Proteome for Comprehensive Proteomics
Data from ProteomeXchange, PXD ID: PXD000900. Data file: HeLa_ArgC-Elastase_CID_2.mgf. Published as part of Mol Cell Proteomics. 2014 Apr 2 . From the Abstract: {{i}} ... In an attempt to improve protein sequence coverage and to target regions of proteins that do not generate useful tryptic peptide...
ORGANISM(S): Homo_sapiens_viruses, Human_female 
GPM11210026998 | GPMDB
Resource Reanalysis 
OtherConfetti: a multiprotease map of the HeLa proteome for comprehensive proteomics.
Data from ProteomeXchange, PXD ID: PXD001258. Experiment: Hela_Try_80pcACN_CID_3xload, file: Hela_Try_80pcACN_CID_3xload_3_20140722.mzXML. Information serves as a correction to Mol Cell Proteomics. 2014 Jun;13(6):1573-84 . From ProteomeXchange: {{i}} Contains corrected HeLa SAX fractionated dataset...
ORGANISM(S): Homo_sapiens_viruses, Human_female 
GPM32320002604 | GPMDB
Resource Reanalysis 
ProteomicsFalcon Probe: A High-Pressure and Robust Sampling Interface for Coupling Lossless Liquid Chromatography Injection with In-Situ Nanoliter-Scale Sample Pretreatment
HeLa protein digest and single 293 cell proteome data analyzed by the Falcon probe-based LC-MS analysis system.
ORGANISM(S): Homo Sapiens 
PXD052544 |
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