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In this work, we performed a fully descriptive analysis N- and O- linked glycosylation of SARS-COV-2 S glycoprotein. We investigated that dual-functionalized Ti-IMAC material enable the simultaneous enrichment and separation of neutral and sialyl glycopeptides of a recombinant SARS-CoV-2 S glycoprot...
ORGANISM(S): Homo sapiens (Human) 
2022-01-11 | PXD026450 | Pride
Protein phosphorylation is one of the most common post-translational modifications (PTMs), which is involved in many important physiological functions. Understanding protein phosphorylation at molecular level is critical to decipher its relevant biological processes and signaling networks. Mass spec...
ORGANISM(S): Homo sapiens (Human) 
2023-10-23 | PXD037549 | Pride
We describe the application of magnetic TiO2 and Ti-IMAC for uniform automated phosphopeptide enrichment. Combining hyper-porous magnetic microspheres with a magnetic particle-handling robot enables rapid (45 minutes), reproducible (r2 = 0.80) and high-fidelity (> 90% purity) phosphopeptide purifica...
ORGANISM(S): Mus musculus (Mouse) 
2014-09-22 | PXD000892 | Pride
Data from ProteomeXchange, PXD ID: PXD000892. File: FIG5_1_KRAS_G12D_3.msf.mgf. Published as part of Anal Chem. 2014 Sep 18 . From the Abstract: {{i}} Reproducible, comprehensive phosphopeptide enrichment is essential to study phosphorylation-regulated processes. Here, we describe the application o...
ORGANISM(S): Mus_musculus_viruses, Mouse 
Fe-IMAC columns for robust and reproducible phosphopeptide ernichment, comparison to TiO2 batch and Ti-IMAC tip enrichment, large scale phosphoproteomics coupling Fe-IMAC column pre-enrichment to subsequent hSAX separation
ORGANISM(S): Homo sapiens (Human) 
2015-08-19 | PXD001060 | Pride
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