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ProteomicsFe-IMAC column based phospho enrichment
Fe-IMAC columns for robust and reproducible phosphopeptide ernichment, comparison to TiO2 batch and Ti-IMAC tip enrichment, large scale phosphoproteomics coupling Fe-IMAC column pre-enrichment to subsequent hSAX separation
ORGANISM(S): Homo sapiens (Human) 
2015-08-19 | PXD001060 | Pride
Hsax Tio2 Ti-imac Ptm Phospho Fe-imac 
OtherComprehensive and reproducible phosphopeptide enrichment using iron immobilized metal ion affinity chromatography (Fe-IMAC) columns.
Data from ProteomeXchange, PXD ID: PXD001060. File: 00730_A11_P005652_S00_T01_R1.mzml. Published as part of Mol Cell Proteomics. 2015 Jan;14(1):205-15 . From the Abstract: {{i}} ... Current offline enrichment approaches using TiO(2), Ti, and Fe immobilized metal ion affinity chromatography (IMAC) m...
ORGANISM(S): Homo_sapiens_viruses, Human 
GPM32310023168 | GPMDB
Resource Reanalysis 
UnknownComprehensive and reproducible phosphopeptide enrichment using iron immobilized metal ion affinity chromatography (Fe-IMAC) columns.
Not available
S-EPMC4288255 | biostudies-literature
ProteomicsRobust, sensitive and automated phosphopeptide enrichment optimized for low sample amounts applied to primary hippocampal neurons
Due to the low stoichiometry of protein phosphorylation, targeted enrichment prior to LC-MS/MS analysis is still essential. The trend in phosphoproteome analysis is shifting towards an increasing number of biological replicates per experiment, ideally starting from very low sample amounts, placing n...
ORGANISM(S): Homo sapiens (Human) Rattus norvegicus (Rat) 
2016-12-14 | PXD005366 | Pride
Phosphoproteomics  quantification  fe(iii)-imac  ti(iv)-imac  tio2  bravo assaymap  phosphopeptide enrichment  sensitivity  egf  hippocampal neurons 
ProteomicsDefeating major contaminants in Fe3+-IMAC phosphopeptide enrichment
Optimized sample preparation workflow for Fe-IMAC phosphopeptide enrichment.
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2018-02-19 | PXD008289 | Pride
Human E.coli 
ProteomicsHuman NEK10 knockout airway ALI Fe-IMAC-MSMS
We identified inactivating mutations in NEK10, a poorly characterized human protein kinase, in a novel human bronchiectasis syndrome. In order to understand effects of loss of its function on the airway phosphoproteome, NEK10 was CRISPR/Cas9 targeted in human airway air-liquid interface (ALI) cultu...
ORGANISM(S): Homo sapiens (Human) 
2020-01-09 | PXD016600 | Pride
Human Nek10 Phosphoproteomics Airway 
ProteomicsZirconium(IV)-IMAC for phosphopeptide enrichment in phosphoproteomics
IMAC and MOAC magnetic microsphere materials were tested with ug amounts of protein extracts from HepG2/C3A cells. We compared Zirconium (IV) IMAC (Zr-IMAC) magnetic microspheres to commonly used Titanium (IV) IMAC (Ti-IMAC) and TiO2 magnetic microspheres for phosphopeptide enrichment from simple an...
ORGANISM(S): Homo sapiens (Human) 
2020-11-26 | PXD018273 | Pride
Affinity enrichment Moac Imac Mass spectrometry Protein phosphorylation Functional proteomics Cell signalling Magnetic particles 
ProteomicsAutomated Phosphopeptide Enrichment for Gram Positive Bacteria
Protein phosphorylation in prokaryotes has gained more attention in recent years as several studies linked it to regulatory and signalling functions indicating an importance similar to protein phosphorylation in eukaryotes. Studies on bacterial phosphorylation have so far been conducted using manual...
ORGANISM(S): Listeria monocytogenes EGD 
2021-08-26 | PXD025459 | Pride
Bravo assaymap  fe(iii)-imac  tio2  automation  phosphopeptide enrichment  phosphoproteomics  quantification  listeria monocytogenes  bacillus subtilis  streptococcus pyogenes 
ProteomicsA Suspension Trapping-based Sample Preparation Workflow for Sensitive Plant Phosphoproteomics
Plant phosphoproteomics reveals a global view of phosphorylation-mediated signaling in plants, but it remains demanding a high-throughput method with detection sensitivity and quantification accuracy. In general, the indispensable protein precipitation step requiring for interferences removal limite...
ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress) 
2023-08-28 | PXD039933 | JPOST Repository
ProteomicsLabel-Free Phosphoproteomics Profiling of Fission Yeast Schizosaccharomyces pombe
The phosphorylation of proteins modulates various functions of proteins and plays an important role in regulation of cell signaling. In the recent years, the label-free quantitative (LFQ) phos-phoproteomics has become the powerful tool to analyze the phosphorylation of proteins within the complex sa...
ORGANISM(S): Schizosaccharomyces pombe 927 
2021-02-12 | PXD023818 | Pride
Pgc Schizosaccharomyces pombe Sax Lfq phosphoproteomics 
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