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ProteomicsFe-IMAC column based phospho enrichment
Fe-IMAC columns for robust and reproducible phosphopeptide ernichment, comparison to TiO2 batch and Ti-IMAC tip enrichment, large scale phosphoproteomics coupling Fe-IMAC column pre-enrichment to subsequent hSAX separation
ORGANISM(S): Homo sapiens (Human) 
2015-08-19 | PXD001060 | Pride
Hsax Tio2 Ti-imac Ptm Phospho Fe-imac 
UnknownHydrophilic strong anion exchange (hSAX) chromatography for highly orthogonal peptide separation of complex proteomes.
Not available
S-EPMC3679572 | biostudies-literature
ProteomicsCrosslinking Interaction Network of Human Mitochondria
Isolated human mitochondria underwent DSSO crosslinking. Following treatment, the mitochondria were separated into soluble and insoluble fractions. Both fractions were then subjected to multidimensional fractionation, including SCX and hSAX. The fractionated peptides were subsequently analyzed using...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD052832 | Pride
Lc-msms Hsax Fractionation Scx Dsso Mitochondria 
UnknownPhytotoxic Metabolites from <i>Hyptis</i> Species: Chemical Profiling and Bioherbicide Activity Against <i>Amaranthus</i>.
Not available
S-EPMC12572983 | biostudies-literature
UnknownPeptide Retention in Hydrophilic Strong Anion Exchange Chromatography Is Driven by Charged and Aromatic Residues.
Not available
S-EPMC5937359 | biostudies-literature
ProteomicsA Proteomic Chronology of Gene Expression through the Cell Cycle in Human Myeloid Leukemia Cells.
Technological advances have enabled the analysis of cellular protein and RNA levels with unprecedented depth and sensitivity, allowing for an unbiased re-evaluation of gene regulation during fundamental biological processes. Here, we have chronicled the dynamics of protein and mRNA expression levels...
ORGANISM(S): Homo sapiens (Human) 
2014-03-12 | PXD000678 | Pride
Hsax Cell cycle Human Fasp Nb4 
UnknownEvaluating the use of HILIC in large-scale, multi dimensional proteomics: Horses for courses?
Not available
S-EPMC4708065 | biostudies-literature
ProteomicsLabel-Free Phosphoproteomics Profiling of Fission Yeast Schizosaccharomyces pombe
The phosphorylation of proteins modulates various functions of proteins and plays an important role in regulation of cell signaling. In the recent years, the label-free quantitative (LFQ) phos-phoproteomics has become the powerful tool to analyze the phosphorylation of proteins within the complex sa...
ORGANISM(S): Schizosaccharomyces pombe 927 
2021-02-12 | PXD023818 | Pride
Pgc Schizosaccharomyces pombe Sax Lfq phosphoproteomics 
ProteomicsLapatinib action and resistance in breast cancer
We used an established cell line model of lapatinib resistance and employed explorative mass spectrometry to profile the proteome, kinome and phosphoproteome changes in an effort to systematically investigate initial inhibitor response and concomitant kinome reprogramming and signaling rewiring in r...
ORGANISM(S): Homo sapiens (Human) 
2017-02-27 | PXD004981 | Pride
Metabolomics Mass spectrometry Phosphoproteomics Breast cancer Drug resistance 
ProteomicsPharmacoproteomic characterisation of human colon and rectal cancer - CRC65 Full Proteomes
Most molecular cancer therapies act on protein targets but data on the proteome status of patients and cellular models are only beginning to emerge. Here, we profiled the proteomes of 65 colorectal cancer (CRC) cell lines to a depth of >10,000 proteins using mass spectrometry. Integration with prote...
ORGANISM(S): Homo sapiens (Human) 
2017-12-12 | PXD005354 | Pride
Personalised medicine Integrated full proteome subtypes Crc65 Mass spectrometry Correlation of mrna and protein abundance Patient stratification Consensus molecular subtypes Cptac Quasar2 Kinobeads 
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