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MetabolomicsHeterologous expression and detection of Apratoxins in E. coli
Not available
ORGANISM(S): Escherichia coli 
ST000446 | MetabolomicsWorkbench
MetabolomicsComplete pathway elucidation and heterologous reconstitution of Rhodiola salidroside biosynthesis
Salidroside is a bioactive tyrosine-derived phenolic natural product found in medicinal plants under the Rhodiola genus. In addition to their roles in traditional medicine as anti-fatigue and anti-anoxia adaptogens, Rhodiola total extract and salidroside have also displayed medicinal properties as a...
2018-01-25 | MTBLS566 | MetaboLights
MetabolomicsMonitoring recombinant protein expression in bacteria by rapid evaporative ionisation mass spectrometry

RATIONALE: There is increasing interest in methods of direct analysis mass spectrometry that bypass complex sample preparation steps.

METHODS: One of the most interesting new ionisation methods is rapid evaporative ionisation mass spectrometry (REIMS) in wh...

2022-01-28 | MTBLS957 | MetaboLights
TranscriptomicsRNA-seq of coding RNA of Pichia pastoris GS115 to determine the effect of inducing heterologous expression of human lysozyme (HuLy) variants
To determine the effect of inducing heterologous expression of human lysozyme (HuLy) variants in Pichia pastoris
ORGANISM(S): Pichia pastoris 
2013-09-12 | E-MTAB-1126 | biostudies-arrayexpress
MetabolomicsGNPS Mara1 Heterologous expression in S. albidoflavus J1074
Heterologous expression of Mara1 in S. ablidoflavus J1074. Mara1 is from S. mirabilis P8-A2
ORGANISM(S): Streptomyces Albidoflavus (ncbitaxon:1886) 
2024-01-26 | MSV000093927 | GNPS
ProteomicsCharacterizing heterologous protein burden in Komagataella phaffii
In this study, we explore the molecular response of various K. phaffii strains that produce either the human insulin precursor or Mambalgin-1, examining changes in transcription and changes in intra- and extracellular protein levels. Our findings provide valuable insights into the molecular mechanis...
ORGANISM(S): Komagataella pastoris (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris) 
2025-03-12 | PXD055382 | Pride
Komagataella phaffii  heterologous protein production  burden  secretomics  continuous cultivation 
ProteomicsMaximizing Heterologous Expression of Engineered Type I Polyketide Synthases: Investigating Codon Optimization Strategies
Type I polyketide synthases (T1PKSs) hold an enormous potential as a rational production platform for the biosynthesis of speciality chemicals. However, despite the great progress in this field, the heterologous expression of PKSs remains a major challenge. One of the first measures to improve heter...
ORGANISM(S): Corynebacterium glutamicum Escherichia coli Pseudomonas putida KT2440 
2023-09-01 | PXD042749 | Pride
Codon optimization Heterologous expression Polyketide synthases 
TranscriptomicsThe heat shock response of killifish (Fundulus heteroclitus): candidate gene and heterologous microarray approaches.
Subspecies of the Atlantic killifish, Fundulus heteroclitus, differ in their maximum thermal tolerance. To determine whether there is a link between the heat shock response (HSR) and maximum thermal tolerance, we exposed 20ºC acclimated killifish from these subspecies to a 2hr heat shock at 34ºC ...
ORGANISM(S): Fundulus heteroclitus 
2010-06-20 | E-GEOD-18792 | biostudies-arrayexpress
ProteomicsHeterologous Expression of MBP-AtABC1K6-His in E. coli
Heterologous E. coli expression of AtABC1K6 tagged with maltose binding protein (MBP) and 6xHis tags revealed a spontaneous cleavage event of the recombinant protein during purification. Resulting bands were resolved on SDS-PAGE and excised for MS/MS identification. Mass spec-based identification of...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-03-02 | PXD030384 | Pride
Coq8 Kinase Spontaneous cleavage Ubib Abc1 
ProteomicsOptimization of a heterologous pathway for protocatechuate catabolism in Escherichia coli
Optimization of a heterologous pathway for protocatechuate catabolism in Escherichia coli
ORGANISM(S): Escherichia Coli (ncbitaxon:562) 
2017-05-30 | MSV000081122 | MassIVE
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