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TranscriptomicsA Basic Domain in the Histone H2B N-terminal Tail is Important for Nucleosome Assembly by FACT
Nucleosome assembly in vivo requires assembly factors, such as histone chaperones, to bind to histones and mediate their deposition onto DNA. In yeast, the essential histone chaperone FACT (FAcilitates Chromatin Transcription) functions in nucleosome assembly and H2A-H2B deposition during transcript...
ORGANISM(S): Saccharomyces cerevisiae 
2016-08-10 | E-GEOD-80831 | biostudies-arrayexpress
TranscriptomicsFACT and Spt6 Prevent Cryptic Transcription by Impeding H2A.Z Loading in Gene Bodies
H2A.Z is a highly conserved histone variant involved in several key nuclear processes. It is incorporated into promoters by SWR-C-related chromatin remodeling complexes, but whether it is also actively excluded from non-promoter regions is not clear. Here, we provide genomic and biochemical evidence...
ORGANISM(S): Saccharomyces cerevisiae 
2015-05-12 | E-GEOD-62880 | biostudies-arrayexpress
ProteomicsThe CENP-T/-W complex is a binding partner of the histone chaperone FACT
The CENP-T/-W histone fold complex, as an integral part of the inner kinetochore, is essential for building a proper kinetochore at the centromere in order to direct chromosome segregation during mitosis. Notably, CENP-T/-W is not inherited at centromeres and new deposition is absolutely required at...
ORGANISM(S): Homo sapiens (Human) 
2016-06-14 | PXD004099 | Pride
Centromere Cenp Mitosis Histone chaperone 
ProteomicsN-terminus of Spt16 anchors FACT to Mcm2 for parental histone recycling
Parental histone recycling is essential for the restoration of chromatin-based epigenetic information during chromatin replication; however, the specific mechanisms underlying the local recycling of parental histones remain poorly understood. Here, we reveal an unexpected role of the Spt16-N domain ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-01-26 | PXD043392 | Pride
Mcm2-7 Fact，parental histone recycling 
GenomicsThe histone chaperone FACT facilitates heterochromatin spreading through regulation of histone turnover and H3K9 methylation states
The histone chaperone FACT facilitates heterochromatin spreading through regulation of histone turnover and H3K9 methylation states
PRJNA730872 | ENA
GenomicsGlutamine methylation on Histone H2A is an RNA Polymerase I dedicated modification
Nucleosomes are decorated with numerous post-translational modifications capable of influencing many DNA processes. Here, we describe a new class of modification, methylation of glutamine, occurring on yeast histone H2A at position 105 (Q105) and human H2A at Q104. We identify Nop1 as the methyltran...
ORGANISM(S): Saccharomyces cerevisiae 
2014-01-21 | E-MTAB-1447 | biostudies-arrayexpress
ProteomicsPhosphorylation of the FACT histone chaperone subunit SPT16 modulates Arabidopsis development and affects chromatin at RNA polymerase II transcriptional start sites
The heterodimeric histone chaperone FACT consisting of SSRP1 and SPT16 contributes to dynamic nucleosome rearrangements during various DNA-dependent processes including transcription. In search of post-translational modifications that may regulate the activity of FACT, SSRP1 and SPT16 were isolated ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-06-09 | PXD028534 | Pride
Arabidopsis Fact complex Acetylation Phosphorylation 
UnknownStructure of the human histone chaperone FACT Spt16 N-terminal domain.
Not available
S-EPMC4741192 | biostudies-literature
UnknownThe histone chaperone FACT facilitates heterochromatin spreading by regulating histone turnover and H3K9 methylation states.
Not available
S-EPMC8608617 | biostudies-literature
ProteomicsPyruvate facilitates FACT-mediated γH2AX loading to chromatin and promotes the radiation resistance of glioblastoma
DNA repair confers the resistance of tumor cells to DNA-damaging anticancer therapies, while how reprogrammed metabolism in tumor cells contributes to such process remains poorly understood. Pyruvate kinase M2 isoform (PKM2) catalyzes the conversion of phosphoenolpyruvate to pyruvate and regulates t...
ORGANISM(S): Homo Sapiens Escherichia Coli 
2021-11-18 | PXD029790 |
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