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TranscriptomicsChromatin immunoprecipitation of human erythroleukemia cell line K-562 with H3K4me2, H3K4me3, H3ac, H4ac, Histone H2B and Histone H3 antibodies using an ENCODE array
ENCODE ChIP-chip study using human myelogenous leukemia cell line K-562 and anti histone H3K4me2 (Abcam; ab7766); H3K4me3 (Abcam; ab8580); H3ac (Upstate; 06-599); H4ac (Upstate; 06-866); Histone H2B (Abcam: ab1790) and Histone H3 (Abcam: ab1791) antibodies. Each antibody experiment was conducted in ...
ORGANISM(S): Homo sapiens 
2006-09-01 | E-TABM-140 | biostudies-arrayexpress
ProteomicsProteomics- and BRET-screens identify SPRY2 as interactor of active Ras the impacts on its membrane organisation
K-Ras is regulated and active in nanoscale proteo-lipid domains of the plasma membrane. Few regulators of K-Ras membrane organisation have been identified so far. We here combined a primary TurboID-based proximal proteome screen with a secondary BRET-screen to identify nine novel bona fide interacto...
ORGANISM(S): Homo sapiens (Human) 
2025-10-02 | PXD067415 | Pride
Turboid Kras Aplp2 Spry2 Bret 
TranscriptomicsHalobacterium NRC-1 oxygen, light and phoR perturbation
A 50ml culture of Halobacterium NRC-1 knockout strain delta-ura3 with additional in-frame gene deletion of phoR was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard clea...
ORGANISM(S): Halobacterium sp. NRC-1 
2008-01-03 | E-GEOD-7730 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 oxygen, light and boa1 perturbation
A 50ml culture of Halobacterium NRC-1 knockout strain delta-ura3 with additional in-frame gene deletion of boa1 was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard clea...
ORGANISM(S): Halobacterium sp. NRC-1 
2008-01-03 | E-GEOD-7723 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 oxygen, light and boa4 perturbation
A 50ml culture of Halobacterium NRC-1 knockout strain delta-ura3 with additional in-frame gene deletion of boa4 was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard clea...
ORGANISM(S): Halobacterium sp. NRC-1 
2008-01-03 | E-GEOD-7725 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 General transcription factor perturbation
Halobacterium cells, specified general transcription factor knock-out strains or plasmid expressed 6-His tagged general transcription factor strains, were grown to mid-log phase. RNA extractions were performed using the Stratagene Absolutely RNA Miniprep Kit and RNA quality checked with the Agilent...
ORGANISM(S): Halobacterium sp. NRC-1 
2010-05-27 | E-GEOD-7713 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 oxygen, light and phr1/phr2 perturbation
A 50ml culture of Halobacterium NRC-1 knockout strain delta-ura3 with additional in-frame gene deletions of phr1 and phr2 was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a sta...
ORGANISM(S): Halobacterium sp. NRC-1 
2008-01-03 | E-GEOD-7716 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array for comparison of industrial Escherichia coli strains on growth, transcriptome and proteome level in batch cultivations to identify strain specific features (subexperiment K-12 platform)
The objective of the current study was to evaluate the performance of frequently used E. coli K-12 strains HMS174 and RV308 in the context of industrial cultivations. To meet this objective, we carried out batch cultivations with high-glucose media in computer-controlled bioreactors and applied tran...
ORGANISM(S): Escherichia coli 
2013-08-19 | E-MARS-20 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 oxygen, light and ura3 perturbation
A 50ml culture of Halobacterium NRC-1 knockout strain delta-ura3 was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard clear flask under constant standard incubator illu...
ORGANISM(S): Halobacterium sp. NRC-1 
2010-05-26 | E-GEOD-7715 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 oxygen and light perturbation
A 50ml culture of Halobacterium NRC-1 was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard clear flask under constant standard incubator illumination, respectively. Low...
ORGANISM(S): Halobacterium sp. NRC-1 
2010-05-26 | E-GEOD-7729 | biostudies-arrayexpress
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