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TranscriptomicsSingle cell RNA-seq of isolated human foetal L/M-opsin cone photoreceptor populations labelled using the AAV2/9.pR2.1.GFP reporter
Human cone photoreceptors represent a rare cell population of the human retina and are largely outnumbered by other retinal cell types. Despite their rarity, cone photoreceptors are critical for optimal daylight vision and their loss has the greatest impact on sight during the progression of retinal...
ORGANISM(S): Homo sapiens 
2018-04-20 | E-MTAB-6058 | biostudies-arrayexpress
TranscriptomicsRNA-seq of isolated human foetal and hiPSC L/M-opsin cone photoreceptor populations labelled using the AAV2/9.pR2.1.GFP reporter
The loss of cone photoreceptor cells, which are critical for optimal daylight vision, have the great impact on vision during retinal degenerations. Retinal differentiation of human induced pluripotent stem cell (hiPSC) sources could provide a renewable source of cone photoreceptors towards developin...
ORGANISM(S): Homo sapiens 
2018-04-20 | E-MTAB-6057 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human irradiated MOLT4 cells to measure measure of degradation rates by actinomycin D
MOLT4 cells in log phase (1x106 cells/ml) were irradiated with 4 Gy at room temperature, at a dose rate of 2.45 Gy/minute with a 137Cs ?-irradiator. After 4 hours, actinomycin D (5 ?M) was added to block transcription. Aliquots of culture were removed at hourly intervals and RNA was extracted (Triz...
ORGANISM(S): Homo sapiens 
2009-06-30 | E-MEXP-2179 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human MOLT4 cells after NFkB inactivation
MOLT4 cells in log phase (1x106 cells/ml) were pre-treated for 2 hours with the I?B? phosphorylation inhibitor BAY 11-7082 (Calbiochem) at 5?M, and the control samples with corresponding volume of DMSO. Cells were then irradiated with 4 Gy at room temperature, at a dose rate of 2.45 Gy/minute with a...
ORGANISM(S): Homo sapiens 
2009-06-30 | E-MEXP-2177 | biostudies-arrayexpress
TranscriptomicsTranscription profiling time series of gene expression following irradiation to identify P53 activity
The purpose of the experiment was to generate a time course of gene expression following irradiation. The goal was then to model this data to extract hidden variables - chiefly, the activity profiles of the p53 transcription factor. Using this information the aim was to predict which transcripts cha...
ORGANISM(S): Homo sapiens 
2006-03-01 | E-MEXP-549 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of liver from rats treated with triiodothyronine at 0.1ug or 5ug after 1 or 3 hours
A single dose of 5 ug of Triiodothyronine (T3) was delivered subcutaneously to adult rats to stimulate hepatocyte proliferation. This was compared to rats treated without T3, or with a a submitogenic dose (0.1 ug). Livers were isolated and harvested at 1 h and 3 h post administration. A comparison w...
ORGANISM(S): Rattus norvegicus 
2007-12-12 | E-MEXP-1077 | biostudies-arrayexpress
TranscriptomicsEffective transplantation of photoreceptor precursor cells selected via cell surface antigen expression.
Microarray analysis of postnatal NrlGFP-expressing rod photoreceptor precursors. NrlGFP positive versus NrlGFP negative at postnatal day 4
ORGANISM(S): Mus musculus 
2013-06-07 | E-MEXP-3922 | biostudies-arrayexpress
TranscriptomicsPhotoreceptor precursors derived from 3D cultures of embryonic stem cells mature and integrate in degenerate retina
To isolate photoreceptor precursors fit for transplantation, we adapted a 3D differentiation protocol that generates neuroretina from mouse ES cells. We used an adeno-associated viral vector (pseudotype2/9)carrying a GFP reporter under the control of a Rhodopsin promoter (AAV2/9.Rhop.GFP)to select r...
ORGANISM(S): Mus musculus 
2013-07-17 | E-MEXP-3921 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of ataxin-null (Atxn-null) vs. wild type mice to investigate spinocerebellar ataxia type 1 (SCA1)
Ataxin 1 (Atxn1) is a protein of unknown function associated with cerebellar neurodegeneration in spinocerebellar ataxia type 1 (SCA1). SCA1 is caused by an expanded polyglutamine within Atxn1 by gain-of-function mechanisms. Lack of Atxn1 in mice triggers motor deficits in the absence of neurodegene...
ORGANISM(S): Mus musculus 
2006-12-01 | E-MEXP-886 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of human paediatric medulloblastoma samples and normal cerebellum to investigate the occurance of alternative splicing
Alternative pre-mRNA splicing critically contributes to the generation of protein variety in a tissue- and development-specific manner. Alterations in the normal pathways of alternative splicing (AS) have been associated with the growth and maintenance of several tumour types, and have been indicate...
ORGANISM(S): Homo sapiens 
2011-01-01 | E-MTAB-292 | biostudies-arrayexpress
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