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MetabolomicsReal-world neonicotinoid mixture exposure reveals synergistic cytotoxicity and divergent pathway vulnerabilities in human cell lines

In this study, five human cell lines (A2780, Chang liver, HEK293T, HeLa, and HepG2) were used as models and exposed to mixtures of neonicotinoids (NEOs) and their metabolites. The mixture concentrations were prepared based on human biomonitoring data to reflect the actual detected levels of NEOs ...

2026-06-22 | MTBLS13896 | MetaboLights
ProteomicsMspire-Simulator: LC-MS shotgun proteomic simulator for creating realistic gold standard data - HEK cell lysate
The most important step in any quantitative proteomic pipeline is feature detection (aka peak picking). However, generating quality hand-annotated data sets to validate the algorithms, especially for lower abundance peaks, is nearly impossible. An alternative for creating gold standard data is to si...
ORGANISM(S): Homo sapiens (Human) 
2020-01-24 | PXD000477 | Pride
Human Ms-simulator Hek-293t Fasp 
ProteomicsTargeted Proteomics Reveals Quantitative Differences in Low Abundance Glycosyltransferases of Patients with Congenital Disorders of Glycosylation
Protein glycosylation is an essential posttranslational modification in all domains of life. Its impairment in human can result in severe diseases named Congenital Disorders of Glycosylation (CDGs). Most of the glycosyltransferases (GTs) responsible for proper glycosylation are polytopic membrane p...
ORGANISM(S): Homo Sapiens 
2024-01-30 | PXD048072 | panorama
ProteomicsA novel differential ion mobility interface expands the depth of proteome coverage and the sensitivity of multiplex proteomic measurements
We present a new high field asymmetric waveform ion mobility spectrometry (FAIMS) interface that can be coupled to the Orbitrap Tribrid mass spectrometers. The interface provides several advantages over previous generations of FAIMS devices including ease of operation, robustness, and high ion trans...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-04-19 | MSV000082293 | MassIVE
ProteomicsLC-MS/MS for identification on ADAR1p110 the sites of ubiquitin conjugation
Adenosine-to-inosine (A-to-I) RNA editing catalyzed by ADAR1 plays essential roles in the regulation of diverse molecular and cellular processes both under physiological conditions and in the disease states, including cancer. However, mechanisms governing the protein abundance and activity of ADAR1,...
ORGANISM(S): Homo sapiens (Human) 
2022-05-29 | PXD025325 | Pride
Human embryonic kidney hek-293t cells Lc-ms/ms 
ProteomicsHuman HEK293T and Pig cardiac top-down proteomes from Bruker maXis II
These data, derived from PXD019368 (PMID 33231430) and PXD010825 (PMID 30988469) represent a test of deconvolution algorithms for top-down proteomics experiments on the Bruker maXis II Q-TOF. Three LC-MS/MS experiments are available from pig cardiac tissue in both raw .d directories and in deconvol...
ORGANISM(S): Sus Scrofa (ncbitaxon:9823) Homo Sapiens (ncbitaxon:9606) 
2022-04-13 | MSV000089252 | MassIVE
ProteomicsAssessing the role of trypsin in quantitative plasma- and single-cell proteomics towards clinical application
Mass spectrometry-based bottom-up proteomics is rapidly evolving and routinely applied in large biomedical studies. Proteases are a central component of every bottom-up proteomics experiment, digesting proteins into peptides. Trypsin has been the most widely applied protease in proteomics, due to it...
ORGANISM(S): Homo Sapiens 
2023-08-29 | PXD042450 | panorama
ProteomicsADAR1 interactome analysis, LC-MS/MS
Adenosine-to-inosine (A-to-I) RNA editing catalyzed by ADAR1 plays essential roles in the regulation of diverse molecular and cellular processes both under physiological conditions and in the disease states, including cancer. However, mechanisms governing the protein abundance and activity of ADAR1,...
ORGANISM(S): Homo sapiens (Human) 
2022-05-29 | PXD025338 | Pride
Human embryonic kidney hek-293t cells lc-ms/ms 
ProteomicsPotential binding partners of NUDT21 in 293T and TE-1 cells
To explore the role of NUDT21 in the progression of ESCC, we performed NUDT21 immunoprecipitation in 293T and TE1 cells following LC-MS/MS analysis.
ORGANISM(S): Homo Sapiens 
2024-12-16 | PXD058885 |
ProteomicsFMRP immunoprecipitation using human HEK293T cells
To define FMRP-interacting proteins in human HEK293T cells, we performed FMRP immunoprecipitation followed by LC-MS/MS analysis.
ORGANISM(S): Homo Sapiens (human) 
2024-10-01 | PXD056408 | JPOST Repository
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