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ProteomicsHuman Histone Sulfated Tyrosine Identification
Tyrosine sulfation is a common posttranslational modification in mammals. To date, it has been thought to be limited to secreted and transmembrane proteins, but little is known about tyrosine sulfation on nuclear proteins.To search for tyrosine sulfation on nuclear proteins, we purified the nucleus ...
ORGANISM(S): Homo sapiens (Human) 
2023-07-13 | PXD043754 | Pride
Human Lc-ms/ms Histone sulfation Liver 
ProteomicsIdentification of Khib substrates of histone regulated by NAT10
Having established NAT10’s regulatory role in lysine 2-hydroxyisobutyrylation, we aimed to identify the histone Khib sites targetedby NAT10. To this end, we extracted and digested core histones from cells with or without NAT10 depletion, followed by chemical derivatization of amine groups. The resul...
ORGANISM(S): Homo sapiens (Human) 
2026-01-27 | PXD066039 | Pride
Human liver cancer histone hplc-ms/ms 
ProteomicsIsonicotinylation is Isonicotinylation is a histone mark induced by the anti-tuberculosis first-line drug isoniazida histone mark induced by the anti-tuberculosis first-line drug isoniazid
Isoniazid (INH) is the first-line anti-tuberculosis drug used for nearly seventy years. Metabolites of INH showed hepatotoxicity in human and tumorigenicity in rodents. However, mechanism underlying the side effects of INH is elusive. Histone acylation is known to be modulated by intracellular metab...
ORGANISM(S): Homo sapiens (Human) 
2021-08-20 | PXD025490 | Pride
Histone isonicotinyltransferases Histone deisonicotinylases Gene transcription Histone isonicotinylation Chromatin structure 
ProteomicsPathology tissue-quantitative mass spectrometry analysis to profile histone post-translational modification patterns in patient samples
Histone post-translational modifications (hPTMs) generate a complex combinatorial code that has been implicated with various pathologies, including cancer. Dissecting such a code in physiological and diseased states may be exploited for epigenetic biomarker discovery, but hPTM analysis in clinical s...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2015-10-14 | PXD002669 | Pride
Biomarker  epigenetics  ffpe  super-silac  cancer 
ProteomicsSuccinylation dimethyl-labeling quantitative proteomic in liver cancer HepG2 cells
Lysine succinylation (Ksucc) as an evolutionarily conserved and widespread post-translational modification has been identified. However, the mechanism of succinylation modulation remains unclear. Here, we report a histone acetyltransferase is a succinyltransferase, essentially enhancing the tumor gr...
ORGANISM(S): Homo sapiens (Human) 
2020-12-10 | PXD013781 | Pride
Proteomic Succinylation Lc-ms/ms 
ProteomicsUnique structural configuration of EV-DNA primes Kupffer cell-mediated anti-tumor immunity to prevent metastatic progression
Extracellular vesicles (EVs) transport biomolecules that mediate intercellular communication. We previously showed that EVs contain DNA (EV-DNA) representing the entire genome. However, the mechanism of EV-DNA packaging and its role in cancer remains elusive. Here, we demonstrate that the majority o...
ORGANISM(S): Homo sapiens (Human) 
2024-09-23 | PXD056104 | Pride
Histone Ptms Ev-dna Kupffer 
ProteomicsGenetic and pharmacologic inhibition of Keap1 dampens the pro-fibrotic program in human hepatic stellate cells
The increasing prevalence of chronic liver diseases (CLDs) is a global health concern. CLDs can cause fibrosis, and there is an urgent need for antifibrotic agents. The primary driver of fibrogenesis, transforming growth factor beta 1 (TGF-β1), activates hepatic stellate cells (HSCs), which are resp...
ORGANISM(S): Homo sapiens (Human) 
2026-09-24 | PXD076470 | Pride
Ecm Fibrosis Liver Nrf2 Anti-fibrotic drugs Antioxidant response 
ProteomicsSystematic investigation of dose-dependent protein thermal stability changes to uncover the mechanisms of the pleiotropic effects of metformin
Metformin is the most popular drug to treat type II diabetes. Besides lowering blood sugar, it is proven to have more beneficial effects, including extending life expectancy. However, the underlying mechanisms remain largely elusive. Here, we systematically studied the proteome thermal stability cha...
ORGANISM(S): Homo sapiens (Human) 
2024-01-11 | PXD039382 | Pride
Thermal proteome profiling Metformin Proteome integral solubility alteration assay 
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