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ProteomicsSubzero hydrogen-deuterium exchange CE-MS
We have performed in solution hydrogen exchange (HDX) on peptide standards and bovine hemoglobin with subsequent quenching, pepsin digestion, and cold capillary electrophoretic separation coupled with mass spectrometry (MS) detection for the benchmarking of a lab built HDX-MS platform. Though neutra...
ORGANISM(S): Bos taurus (Bovine) 
2022-12-22 | PXD034245 | Pride
Ce-ms Hdx Hemoglobin 
ProteomicsON-TISSUE DIRECT MONITORING OF GLOBAL HYDROGEN/DEUTERIUM EXCHANGE BY MALDI MASS SPECTROMETRY: TDXMS
Hydrogen/deuterium exchange mass spectrometric methods for protein structural analysis are conventionally performed in solution. We present Tissue Deuterium Exchange Mass Spectrometry (TDXMS), a method to directly monitor deuterium uptake on tissue, as a means to better approximate the deuterium exc...
ORGANISM(S): Rattus norvegicus (Rat) 
2016-08-15 | PXD004630 | Pride
Tissue Mass spectrometry In situ analysis H/d exchange 
ProteomicsEpitope Mapping of Human Polyclonal Antibodies to the fHbp Antigen of a Neisseria Meningitidis Vaccine by Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS)
Antigen-antibody interactions play a key role in the immune response post vaccination and the mechanism of action of antibody-based biopharmaceuticals. 4CMenB is a multicomponent vaccine against Neisseria meningitidis serogroup B in which factor H binding protein (fHbp) is one of the key antigens. I...
ORGANISM(S): Homo sapiens (Human) 
2024-05-24 | PXD046572 | Pride
Polyclonal antibodies Pab Fhbp Hdx-ms 
ProteomicsEpitope Mapping of Human Polyclonal Antibodies to the fHbp Antigen of a Neisseria Meningitidis Vaccine by Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) - part 2
Antigen-antibody interactions play a key role in the immune response post vaccination and the mechanism of action of antibody-based biopharmaceuticals. 4CMenB is a multicomponent vaccine against Neisseria meningitidis serogroup B in which factor H binding protein (fHbp) is one of the key antigens. I...
ORGANISM(S): Homo sapiens (Human) 
2024-05-22 | PXD047712 | Pride
Pab Fhbp Hdx-ms 
ProteomicsEukaryotic Ribosome GCN2 interaction Hydrogen/Deuterium Exchange Mass Spectrometry
General control nonderepressible 2 (GCN2) phosphorylates eIF2α, regulating translation in response to nutritional stress. Here, we show that mammalian ribosomes are potent GCN2 activators. Hydrogen/deuterium exchange–mass spectrometry (HDX-MS) showed GCN2 interacting with domain II of the uL10 P-sta...
ORGANISM(S): Oryctolagus cuniculus (Rabbit) 
2019-05-24 | PXD013051 | Pride
Ribosome Gcn2 Hdx-ms 
ProteomicsHydrogen-Deuterium Exchange Reveals Catalytically Linked Protein Flexibility in Myoglobin-Mediated Intramolecular C(sp3)-H Activation
Hydrogen-Deuterium Exchange Reveals Catalytically Linked Protein Flexibility
ORGANISM(S): Escherichia coli 
2025-12-29 | PXD071026 | Pride
Myoglobin 
ProteomicsHydrogen-deuterium exchange mass spectrometry of wt Mtr4 with various RNA substrates
Mtr4 is a eukaryotic RNA helicase required for RNA decay by the nuclear exosome. Previous studies have shown how RNA enroute to the exosome threads through the highly conserved helicase core of Mtr4. Mtr4 also contains an arch domain, although details of potential interactions between the arch and R...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-03-07 | PXD031962 | Pride
Hdx Helicase Hdx-ms Mtr4 Bottom-up hdx 
ProteomicsHuman Calcitonin and Amylin receptor HDX-MS
Receptor activity-modifying proteins (RAMPs) regulate the pharmacology, trafficking, and signaling of class B1 G protein-coupled receptors (GPCRs). The calcitonin receptor (CTR) interacts with each of the three RAMPs to form amylin receptors (AMYRs), which display distinct ligand preferences and sig...
ORGANISM(S): Homo sapiens (Human) 
2026-06-08 | PXD068848 | Pride
Calcitonin recepto Hdx-ms Recepror activity modifying protein (ramp) Gpcr Amylin receptor 
ProteomicsDetermination of highly accurate small molecule:protein affinity constants at amino acid resolution using Hydrogen Deuterium Exchange Mass Spectrometry (HDX-MS)
Hydrogen Deuterium Exchange Mass Spectrometry (HDX-MS) has been widely adopted as part of the drug development pipeline, frequently used in both the development of biopharmaceuticals and small molecule compound development to assess target engagement spatially. Frequently, HDX-MS analyses are conduc...
ORGANISM(S): Plasmodium falciparum 58.1 
2026-04-01 | PXD070051 | Pride
ProteomicsAspergillus niger Prolyl endoprotease (An-PEP) for Hydrogen-Deuterium Exchange Mass Spectrometry and Protein Structural Studies
To monitor the structural integrity of therapeutic proteins, hydrogen-deuterium exchange mass spectrometry (HDX-MS) is increasingly utilized in the pharmaceutical industry. The successful outcome of HDX-MS analyses depends on the sample preparation conditions, which involve the rapid digestion of pr...
ORGANISM(S): Escherichia coli 
2017-07-12 | PXD006766 | Pride
Hydrogen-deuterium exchange Hemoglobin Protease Mass spectrometry Aspergillus niger Shorgun proteomics Igg4 
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