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MetabolomicsGCN2 regulates mitochondrial OXPHOS in HSPCs under proliferation conditions.
Not available
ORGANISM(S): Mus musculus 
ST002123 | MetabolomicsWorkbench
ProteomicsEukaryotic Ribosome GCN2 interaction Hydrogen/Deuterium Exchange Mass Spectrometry
General control nonderepressible 2 (GCN2) phosphorylates eIF2α, regulating translation in response to nutritional stress. Here, we show that mammalian ribosomes are potent GCN2 activators. Hydrogen/deuterium exchange–mass spectrometry (HDX-MS) showed GCN2 interacting with domain II of the uL10 P-sta...
ORGANISM(S): Oryctolagus cuniculus (Rabbit) 
2019-05-24 | PXD013051 | Pride
Ribosome Gcn2 Hdx-ms 
TranscriptomicsSelective control of metabolism by the GCN2 eIF2 kinase pathway in Saccharomyces cerevisiae
When eukaryotic cells are deprived of amino acids, uncharged tRNAs accumulate and activate the conserved GCN2 protein kinase. We examine how yeast growth and tRNA charging or aminoacylation is affected during amino acid depletion in the presence and absence of GCN2. tRNA charging is measured using ...
ORGANISM(S): Saccharomyces cerevisiae 
2010-08-01 | E-GEOD-22452 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse Gcn2 wild-type and knockout liver perfused with or without methionine
In eukaryotes, regulation of mRNA translation enables a fast, localized and finely tuned expression of gene products. Within the translation process, the first stage of translation initiation is most rigorously modulated by the actions of eukaryotic initiation factors (eIFs) and their associated pr...
ORGANISM(S): Mus musculus 
2009-11-23 | E-GEOD-11496 | biostudies-arrayexpress
TranscriptomicsUnfolded protein response in wildtype, ire1, gcn4, gcn2
We measured steady-state mRNA levels by microarray hybridization, comparing WT, (delta)ire1, (delta)gcn4, and (delta)gcn2 cells treated with 2 mM DTT for 30 min (by which time the UPR is qualitatively complete) to untreated samples of the same genotype. WT cells were taken as a positive control for ...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-06 | E-GEOD-1688 | biostudies-arrayexpress
TranscriptomicsExamination of gene expression in cerebellum of mouse strains B6J, B6J-nmf205-/-, B6J-Gcn2-/- and B6J-nmf205-/-;Gcn2-/-.
Ribosome stalling during translation has recently been shown to cause neurodegeneration, yet the signaling pathways triggered by stalled elongation complexes are unknown. To investigate these pathways we analyzed the brain of B6J-nmf205-/- mice in which neuronal elongation complexes are stalled at...
ORGANISM(S): Mus musculus 
2016-06-15 | E-GEOD-79929 | biostudies-arrayexpress
ProteomicsGCN2 Adapts Protein Synthesis to Scavenging-Dependent Growth
Compared protein synthesis in Gcn2 WT vs Gcn2 KO murine PDAC cells (KRPC) in amino acid-replete or leucine-free media.
ORGANISM(S): Mus musculus (Mouse) 
2022-10-13 | PXD028143 | Pride
Gcn2 Macropinocytosis Pdac 
TranscriptomicsTranscription profiling of mouse liver to assess the translational response following activation of GCN2 versus PERK - super series
In eukaryotes, regulation of mRNA translation enables a fast, localized and finely tuned expression of gene products. Within the translation process, the first stage of translation initiation is most rigorously modulated by the actions of eukaryotic initiation factors (eIFs) and their associated pr...
ORGANISM(S): Mus musculus 
2009-11-23 | E-GEOD-11685 | biostudies-arrayexpress
ProteomicsGCN2 Restrains ZAKα-Dependent TNFα Production in LPS+IFNγ Stimulated Macrophages
Macrophages orchestrate inflammation through rapid and extensive proteome remodeling, yet the translational programs governing macrophage activation remain poorly defined. Here, we show that inflammatory M(LPS+IFNγ) and reparative M(IL-4) macrophages engage fundamentally distinct translational traje...
ORGANISM(S): Mus musculus (Mouse) 
2026-09-28 | PXD075112 | Pride
Macrophage Zakα Translation Gcn2 Inflammation 
TranscriptomicsActivation of GCN2 by Ribosome Stalling Links Translation Elongation with Translation Initiation
This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
ORGANISM(S): Mus musculus 
2016-06-15 | E-GEOD-79930 | biostudies-arrayexpress
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