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ProteomicsCharting the interactome of PDE3A in human cells using an IBMX based chemical proteomics approach
We attempt to study the interactome of PDEs by synthesizing a broad spectrum PDE-capturing resin based on the non-selective PDE inhibitor 3-isobutyl-1-methylxanthine (IBMX). Chemical proteomics characterization of this resin in HeLa cell lysates led to the capture of several different PDEs. Combinin...
ORGANISM(S): Homo sapiens (Human) 
2016-05-25 | PXD001781 | Pride
Ibmx Mass spectrometry Phosphodiesterase Papaverine Cilostamide Chemical proteomics 
TranscriptomicsGlucocorticoid Signaling Defines a Novel Commitment State during Adipogenesis In Vitro
Differentiation of 3T3-L1 preadipocytes can be induced by a 2-d treatment with a factor "cocktail" (DIM) containing the synthetic glucocorticoid dexamethasone (dex), insulin, the phosphodiesterase inhibitor methylisobutylxanthine (IBMX) and fetal bovine serum (FBS). We temporally uncoupled...
ORGANISM(S): Mus musculus 
2008-07-25 | E-GEOD-11249 | biostudies-arrayexpress
GenomicsGene expressions of IBMX-induced neuron differentiation from placenta-derived multipotent stem cells
Gene expressions of IBMX-induced neuron differentiation from placenta-derived multipotent stem cells
PRJNA586828 | ENA
UnknownForskolin and IBMX Induce Neural Transdifferentiation of MSCs Through Downregulation of the NRSF.
Not available
S-EPMC6393535 | biostudies-literature
TranscriptomicsGene expressions of IBMX-induced neuron differentiation from placenta-derived multipotent stem cells
Little is known about factors that induce stem cells differentiated into astrocyte. Using double cross strategy, we effective narrow down the candidate molecules crucial for astroglial differentiation. By cross comparison the data from transcriptomic and proteomic experiments, we selected 24 candida...
ORGANISM(S): Homo sapiens 
2019-11-01 | GSE139656 | GEO
TranscriptomicsTranscription profiling of mouse C3H10T1/2 cells stimulated to differentiate using IDMB to investigate adipogenesis
Confluent C3H10T1/2 cells stimulated to differentiate using IDMB (1uM BRL49653, 1uM Dexamethasone, 0.5uM IBMX, 10ug/mL Insulin). Expression was examined 24 hours after IDMB treatment. Additionally, the effects of addition of TCDD 48 hours prior to IDMB treatment, and EGF addition concurrent with I...
ORGANISM(S): Mus musculus 
2007-12-14 | E-GEOD-1123 | biostudies-arrayexpress
TranscriptomicsPPARg dependent expression changes during differentiation of 3T3L1 cell into adipocytes
We profiled PPARg dependent gene expression changes during differntiation of 3T3L1 cell using PPARg siRNA 3T3-L1 (Pre-adipocyte) cell line was induced to differentiate using standard adipocyte differentiation media (IBMX, Dex and Insulin) 48hrs post-confluency. RNA was harvested at day -2 (confluent...
ORGANISM(S): Mus musculus 
2010-04-08 | E-GEOD-12929 | biostudies-arrayexpress
ProteomicsQuantitative proteomics of IBMX-induced neuron differentiation from placenta-derived multipotent stem cells
placenta-derived multipotent stem cells (PDMC) were stimulated by IBMX to differentiated into neuronal-like cells. The cells were lyzed, trypsin digested, and dimethyl labled, followed by strong cation exchange fractionation. The peptides were analyzed by nanoLC-MS/MS with Thermo LTQ-Orbitrap XL. Pr...
ORGANISM(S): Homo Sapiens (human) 
2021-08-14 | PXD016085 | JPOST Repository
ProteomicsComprehensive Proteomic Profiling of Converted Adipocyte-like Cells from Normal Human Dermal Fibroblast using Data-Independent Acquisition Mass Spectrometry (DIA-MS)
We applied direct conversion of normal human dermal fibroblast (NHDF) into adipocyte-like cells using adipogenic cocktail containing IBMX, dexamethasone, insulin, and rosiglitazone and confirmed prominent lipid droplet accumulation in the converted cells for proteomic research
ORGANISM(S): Homo sapiens (Human) 
2025-05-13 | PXD050528 | Pride
Adipocyte-like cells Dia-ms Mass spectrometry Proteomics Extracellular proteome Normal human dermal fibroblast Intracellular proteome. 
TranscriptomicsTranscriptome analysis of cyclic AMP (cAMP) response in bovine cumulus cells
Study the effect of forskolin, dipyridamol and IBMX (here called FID) supplementation in the in vitro maturation medium for an incubation of 6 hrs on the transcriptomics of bovine cumulus cells. 3 biological replicates of cumulus cells derived from cumulus oocyte complexes (COCs) supplemented with F...
ORGANISM(S): Bos taurus 
2015-07-01 | E-GEOD-67968 | biostudies-arrayexpress
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