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ProteomicsMitochondrial KMT9 methylates DLAT to control pyruvate dehydrogenase activity, de novo lipogenesis, and prostate cancer growth
Prostate cancer (PCa) growth depends on de novo lipogenesis controlled by the mitochondrial pyruvate dehydrogenase complex (PDC). In this study, we identified lysine methyltransferase (KMT)9 as a novel regulator of PDC activity. KMT9 is localized in mitochondria of PCa cells, but not in mitochondria...
ORGANISM(S): Homo sapiens (Human) 
2024-12-03 | PXD053942 | Pride
Protein methylation Metabolism Mitochondria 
ProteomicsA time-resolved atlas of histone modifications during mitotic entry
Mitotic chromosome formation is essential for faithful chromosome segregation in metazoans. While condensin complexes are critical for the formation of rod-shaped mitotic chromosomes, additional mechanisms—particularly those involving phosphorylation and deacetylation of specific histone residues—ha...
ORGANISM(S): Gallus gallus (Chicken) 
2026-05-13 | PXD066101 | Pride
Chicken Ptms Mitosis Histones Lcms 
ProteomicsDrosophila embryo extract assisted in vitro chromatin assembly in presence of foldamer
Drosophila preblastoderm embryo extract assisted chromatin assembly with or without 16mer DNA mimic. Pull down of chromatin fiber to reveal chromatin binders in the system.
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2023-09-07 | PXD039966 | Pride
Dna mimcs Qexactive hf Lc-ms In vitro chromatin assembly Drosophila embryo extract (drex) 
ProteomicsPulldown with DNA mimicking foldamers in preblastoderm Drosophila embryo extract
16mer and 32mer biotinylated DNA mimics (“foldamers”) bound to streptavidin beads, AP-MS after 1h incubation in Drosophila preblastoderm embryo extract.
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2023-09-07 | PXD040157 | Pride
Dna mimcs Lc-ms Exploris 480 In vitro chromatin assembly Drosophila embryo extract (drex) 
ProteomicsDNA mimicking 32mer foldamers interfere with in vitro chromatin assembly in drosophila embryo extract
Systematic study of the influence of different concentrations of DNA-mimicking foldamers (32mer) on the chromatin-bound bound proteome using an in vitro chromatin assembly extract
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2023-09-07 | PXD040158 | Pride
Dna mimcs Lc-ms Exploris 480 In vitro chromatin assembly Drosophila embryo extract (drex) 
ProteomicsProximity proteomics using AMPL-MS coupled to analysis for histone modification
Eukaryotic chromatin is organised in individual domains, which are defined by their location within the nuclear space and their distinct proteomic compositions. In contrast to cellular organelles surrounded by lipid membranes, the composition of distinct chromatin domains is rather ill described and...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2024-05-29 | PXD044296 | Pride
Histone modification Centromere Protein a-apex2 X-chromosome Biotinylation Chromatin Proximity proteomics 
ProteomicsA quantitative proteomic analysis of in vitro assembled chromatin
The structure of chromatin is critical for many aspects of cellular physiology and is considered to be the primary medium to store epigenetic information. It is defined by the histone molecules that constitute the nucleosome, the positioning of the nucleosomes along the DNA and the non-histone prote...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2016-01-27 | PXD002537 | Pride
Acetylation  affinity proteomics  chromatin function or biology  label-free quantification   macromolecular assemblages 
ProteomicsProximity proteomics using AMPL-MS
Eukaryotic chromatin is organised in individual domains, which are defined by their location within the nuclear space and their distinct proteomic compositions. In contrast to cellular organelles surrounded by lipid membranes, the composition of distinct chromatin domains is rather ill described and...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2024-05-29 | PXD044295 | Pride
Proximity proteomics using ampl-ms 
ProteomicsThe GFZF protein interactome in Drosophila melanogaster 0-12h old embryos
The gfzf gene is essential for viability and it has been identified in several genetic screenings pointed out its importance in different cellular processes. However, its molecular function has not been extensively studied so far. Here we analyse the GFZF protein interactome in Drosophila melanogast...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2019-11-12 | PXD010712 | Pride
Drosophila melanogaster / embryos / gfzf / interactome 
ProteomicsIn vitro chromatin assembly - A quantitative proteomic analysis of in vitro assembled chromatin
Here, we use LC-MSMS and SWATH-MS to describe the kinetics of in vitro assembled chromatin supported by an embryo extract prepared from preblastoderm Drosophila melanogaster embryos. This system allows easy manipulation of distinct aspects of chromatin assembly such as post-translational histone mod...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2016-01-27 | PXD003445 | Pride
Lc-msms Chromatin assembly Drosophila melanogaster 
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