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ProteomicsValidation and invalidation of chemical probes for the human N-myristoyltransferases.
On-target, cell-active chemical probes are of fundamental importance in both chemical and cell biology, whereas the application of poorly-characterised probes often leads to invalid conclusions. Human N-myristoyltransferase (NMT) has attracted increasing interest as a target in cancer and infectious...
ORGANISM(S): Homo sapiens (Human) 
2019-11-14 | PXD012722 | Pride
N-myristoylation Imp-366 (ddd85646) Metabolic tagging Tris-dba palladium Myristic acid alkyne ynmyr Chemical proteomics Imp-1088 D-nmappd (b13) 2-hydroxymyristic acid 
ProteomicsMetabolic labeling of NMT substrates by photoactivatable/clickable myristate probes X3/X8/X10
Efficiency of labelling by photoactivatable/clickable myristate probes X3/X8/X10 is compared to the positive control probe YnMyr. Additionally, negative controls for each probe were obtained by treatment with NMT ihibitor IMP-366 (DDD85464). The resulting data indicates efficient labeling of NMT sub...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD027239 | Pride
Nmt Diazirine Metabolic labeling Myristoylation Photocrosslinking 
ProteomicsIdentification of protein-protein interaction mediated by N-myristoylated proteins using photocrosslinkable clickable myristoylation probes X3 and X10
HeLa cells were treated with myristoylation probes X3 and X10, which incorporate both alkyne and diazirine moieties. Cells were then treated with NMT ihibitor IMP-366 (DDD85464) or DMSO vehicle and UV-irradiated before lysis. The experiment identifies myristoylated proteins through comparison of veh...
ORGANISM(S): Homo sapiens (Human) 
2023-02-04 | PXD029944 | Pride
Diazirine Nmt Photocrosslinking Myristoylaion 
UnknownValidation and Invalidation of Chemical Probes for the Human N-myristoyltransferases.
Not available
S-EPMC6593224 | biostudies-literature
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